Fatty acids regulate Thy-1 antigen mRNA stability in T lymphocyte precursors.

Détails

ID Serval
serval:BIB_76CA8C1BCA75
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Fatty acids regulate Thy-1 antigen mRNA stability in T lymphocyte precursors.
Périodique
European Journal of Biochemistry
Auteur(s)
Déglon N., Wilson A., Desponds C., Laurent P., Bron C., Fasel N.
ISSN
0014-2956 (Print)
ISSN-L
0014-2956
Statut éditorial
Publié
Date de publication
1995
Volume
231
Numéro
3
Pages
687-696
Langue
anglais
Résumé
In this study, we report the effect of fatty acids on the Thy-1 antigen mRNA decay. Low serum and synthetic medium culture conditions were used to demonstrate that fatty acids, which are important metabolites involved as second messengers in signal transduction, also influence the steady-state mRNA level. Detailed analysis demonstrated that polyunsaturated lipids attached to bovine serum albumin, such as linoleic, linolenic, and arachidonic acids, modulate gene expression specifically in the S1A T lymphoma cell line by inducing a 3-5-fold increase in the steady-state Thy-1 mRNA level, concomitant with a twofold increase in cell surface expression. A similar modulation was observed in the immature CD4-CD8- T cell precursors but not in mature thymocytes. Nuclear run-on and transfection experiments indicated that the observed Thy-1 mRNA level is post-transcriptionally regulated and that the presence of the coding region is sufficient for this adaptive response. A mechanism without a requirement for protein kinase C activation, but involving Ca2+ entry, could account for this difference in Thy-1 mRNA stability.
Mots-clé
Animals, Antigens, Thy-1/genetics, Calcium/metabolism, Down-Regulation, Enzyme Activation, Fatty Acids/pharmacology, Gene Expression Regulation/drug effects, Ion Transport, Ionomycin/pharmacology, Kinetics, Mice, Protein Kinase C/metabolism, RNA Processing, Post-Transcriptional, RNA, Messenger/metabolism, T-Lymphocytes/immunology, T-Lymphocytes/metabolism, Tumor Cells, Cultured
Pubmed
Web of science
Création de la notice
24/01/2008 15:02
Dernière modification de la notice
20/08/2019 14:33
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