Macrophage-colony stimulating factor (M-CSF) regulates the expression of fibronectin and its alpha5 integrin receptor in human trophoblasts.

Détails

ID Serval
serval:BIB_75BB2800A083
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Macrophage-colony stimulating factor (M-CSF) regulates the expression of fibronectin and its alpha5 integrin receptor in human trophoblasts.
Périodique
Endocrinology
Auteur(s)
Omigbodun A., Coukos G., Ziolkiewicz P., Wang C.L., Coutifaris C.
ISSN
0013-7227 (Print)
ISSN-L
0013-7227
Statut éditorial
Publié
Date de publication
1998
Volume
139
Numéro
4
Pages
2190-2193
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.Publication Status: ppublish
Résumé
Macrophage-colony stimulating factor (M-CSF, CSF-1) is secreted by human trophoblasts as well as endometrial cells, while its receptor c-fms is abundantly expressed by the extravillous trophoblasts anchoring the placenta to the uterus, suggesting a role for M-CSF at the maternal-fetal interface. We investigated the effect of M-CSF on the expression of fibronectin and its receptor, the alpha5 integrin, in human trophoblasts. Exposure of trophoblasts to M-CSF produced a two to three-fold increase in fibronectin and alpha5 mRNA abundance at both 24 and 72 hours of culture. A dose-dependent increase in cellular fibronectin secretion into the culture medium was detected at both time points. Immunocytochemistry showed co-localization of cellular fibronectin and alpha5 in the cells, suggesting that attachment of trophoblasts to fibronectin is mediated in part by the alpha5beta1 integrin. We conclude that M-CSF increases fibronectin expression and secretion by the human trophoblasts, and up-regulates its specific receptor, the alpha5 integrin. We hypothesize that M-CSF may partake in the autocrine/paracrine mechanisms regulating trophoblast invasion during implantation.
Mots-clé
Antigens, CD/analysis, Antigens, CD/genetics, Cells, Cultured, Embryo Implantation, Female, Fibronectins/analysis, Fibronectins/genetics, Fluorescent Antibody Technique, Indirect, Gene Expression Regulation, Humans, Integrin alphaV, Macrophage Colony-Stimulating Factor/pharmacology, Pregnancy, RNA, Messenger/biosynthesis, Trophoblasts/chemistry, Trophoblasts/metabolism
Pubmed
Web of science
Création de la notice
14/10/2014 12:43
Dernière modification de la notice
03/03/2018 18:24
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