Amazonian Phlebovirus (Bunyaviridae) potentiates the infection of Leishmania (Leishmania) amazonensis: Role of the PKR/IFN1/IL-10 axis.

Détails

ID Serval
serval:BIB_75870B3A26CF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Amazonian Phlebovirus (Bunyaviridae) potentiates the infection of Leishmania (Leishmania) amazonensis: Role of the PKR/IFN1/IL-10 axis.
Périodique
PLoS neglected tropical diseases
Auteur(s)
Rath C.T., Schnellrath L.C., Damaso C.R., de Arruda L.B., Vasconcelos PFDC, Gomes C., Laurenti M.D., Calegari Silva T.C., Vivarini Á.C., Fasel N., Pereira RMS, Lopes U.G.
ISSN
1935-2735 (Electronic)
ISSN-L
1935-2727
Statut éditorial
Publié
Date de publication
06/2019
Peer-reviewed
Oui
Volume
13
Numéro
6
Pages
e0007500
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
Leishmania parasites are transmitted to vertebrate hosts by phlebotomine sandflies and, in humans, may cause tegumentary or visceral leishmaniasis. The role of PKR (dsRNA activated kinase) and Toll-like receptor 3 (TLR3) activation in the control of Leishmania infection highlights the importance of the engagement of RNA sensors, which are usually involved in the antiviral cell response, in the fate of parasitism by Leishmania. We tested the hypothesis that Phlebovirus, a subgroup of the Bunyaviridae, transmitted by sandflies, would interfere with Leishmania infection.
We tested two Phlebovirus isolates, Icoaraci and Pacui, from the rodents Nectomys sp. and Oryzomys sp., respectively, both natural sylvatic reservoir of Leishmania (Leishmania) amazonensis from the Amazon region. Phlebovirus coinfection with L. (L.) amazonensis in murine macrophages led to increased intracellular growth of L. (L.) amazonensis. Further studies with Icoaraci coinfection revealed the requirement of the PKR/IFN1 axis on the exacerbation of the parasite infection. L. (L.) amazonensis and Phlebovirus coinfection potentiated PKR activation and synergistically induced the expression of IFNβ and IL-10. Importantly, in vivo coinfection of C57BL/6 mice corroborated the in vitro data. The exacerbation effect of RNA virus on parasite infection may be specific because coinfection with dengue virus (DENV2) exerted the opposite effect on parasite load.
Altogether, our data suggest that coinfections with specific RNA viruses shared by vectors or reservoirs of Leishmania may enhance and sustain the activation of host cellular RNA sensors, resulting in aggravation of the parasite infection. The present work highlights new perspectives for the investigation of antiviral pathways as important modulators of protozoan infections.
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/06/2019 16:08
Dernière modification de la notice
21/08/2019 5:36
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