Beta 1- and beta 3-subunits can associate with presynthesized alpha-subunits of Xenopus oocyte Na,K-ATPase.

Détails

ID Serval
serval:BIB_7547630A7B18
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Beta 1- and beta 3-subunits can associate with presynthesized alpha-subunits of Xenopus oocyte Na,K-ATPase.
Périodique
Journal of Biological Chemistry
Auteur(s)
Ackermann U., Geering K.
ISSN
0021-9258
Statut éditorial
Publié
Date de publication
06/1992
Peer-reviewed
Oui
Volume
267
Numéro
18
Pages
12911-12915
Langue
anglais
Résumé
Oligomerization of newly synthesized alpha- and beta-subunits is a prerequisite for the structural and functional maturation of Na,K-ATPase. In this study, we have tested the competence of presynthesized alpha- and beta-subunits to assemble into functional enzyme complexes. Antisense oligonucleotides complementary to alpha-mRNA were used to inhibit alpha-subunit synthesis in Xenopus oocytes leaving a presynthesized trypsin-sensitive alpha-subunit pool. beta-Subunits expressed in these oocytes from injected cRNA assembled with the preexisting alpha-subunits, rendered them trypsin-resistant, and permitted the expression of more ouabain binding sites at the plasma membrane. Similarly, presynthesized beta 1- or beta 3-subunits produced in Xenopus oocytes by injection of beta-cRNA and later of specific antisense oligonucleotides were stabilized and transported out of the endoplasmic reticulum when alpha-cRNA was injected into oocytes. These data indicate that alpha- and beta-subunits can insert into endoplasmic reticulum membranes independent of each other in an assembly-competent form and retain their ability for oligomerization after synthesis.
Mots-clé
Animals, Base Sequence, Binding Sites, Cell Membrane/metabolism, DNA, Half-Life, Molecular Sequence Data, Oocytes/enzymology, Ouabain/pharmacology, Protein Conformation, Sodium-Potassium-Exchanging ATPase/chemistry, Sodium-Potassium-Exchanging ATPase/metabolism, Trypsin, Xenopus
Pubmed
Web of science
Création de la notice
24/01/2008 13:28
Dernière modification de la notice
03/03/2018 18:23
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