Arabidopsis glucosinolates trigger a contrasting transcriptomic response in a generalist and a specialist herbivore.

Détails

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Etat: Serval
Version: Author's accepted manuscript
ID Serval
serval:BIB_752A1BE3B46B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Arabidopsis glucosinolates trigger a contrasting transcriptomic response in a generalist and a specialist herbivore.
Périodique
Insect biochemistry and molecular biology
Auteur(s)
Schweizer F., Heidel-Fischer H., Vogel H., Reymond P.
ISSN
1879-0240 (Electronic)
ISSN-L
0965-1748
Statut éditorial
Publié
Date de publication
06/2017
Peer-reviewed
Oui
Volume
85
Pages
21-31
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article
Publication Status: ppublish
Résumé
Phytophagous insects have to deal with toxic defense compounds from their host plants. Although it is known that insects have evolved genes and mechanisms to detoxify plant allochemicals, how specialist and generalist precisely respond to specific secondary metabolites at the molecular level is less understood. Here we studied the larval performance and transcriptome of the generalist moth Heliothis virescens and the specialist butterfly Pieris brassicae feeding on Arabidopsis thaliana genotypes with different glucosinolate (GS) levels. H. virescens larvae gained significantly more weight on the GS-deficient mutant quadGS compared to wild-type (Col-0) plants. On the contrary, P. brassicae was unaffected by the presence of GS and performed equally well on both genotypes. Strikingly, there was a considerable differential gene expression in H. virescens larvae feeding on Col-0 compared to quadGS. In contrast, compared to H. virescens, P. brassicae displayed a much-reduced transcriptional activation when fed on both plant genotypes. Transcripts coding for putative detoxification enzymes were significantly upregulated in H. virescens, along with digestive enzymes and transposable elements. These data provide an unprecedented view on transcriptional changes that are specifically activated by GS and illustrate differential molecular responses that are linked to adaptation to diet in lepidopteran herbivores.

Mots-clé
Animals, Arabidopsis, Butterflies/drug effects, Butterflies/genetics, Butterflies/metabolism, Down-Regulation, Gene Expression Profiling, Gene Expression Regulation, Plant, Glucosinolates/pharmacology, Herbivory, Inactivation, Metabolic/genetics, Insect Proteins/metabolism, Larva/drug effects, Moths/drug effects, Moths/genetics, Moths/metabolism, Sequence Analysis, RNA, Transcriptome/drug effects, Arabidopsis thaliana, Detoxification, Glucosinolates, Heliothis virescens, Insect transcriptome, Pieris brassicae
Pubmed
Web of science
Open Access
Oui
Création de la notice
09/05/2017 18:35
Dernière modification de la notice
08/05/2019 20:30
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