The calcium-binding protein calreticulin is a major constituent of lytic granules in cytolytic T lymphocytes

Détails

ID Serval
serval:BIB_751462C2078F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The calcium-binding protein calreticulin is a major constituent of lytic granules in cytolytic T lymphocytes
Périodique
Journal of Experimental Medicine
Auteur(s)
Dupuis  M., Schaerer  E., Krause  K. H., Tschopp  J.
ISSN
0022-1007 (Print)
Statut éditorial
Publié
Date de publication
01/1993
Volume
177
Numéro
1
Pages
1-7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan 1
Résumé
Cytolytic T lymphocytes (CTL), natural killer cells, and lymphokine-activated killer (LAK) cells are cytolytic cells known to release the cytolytic protein perforin and a family of proteases, named granzymes, from cytoplasmic stores upon interaction with target cells. We now report the purification of an additional major 60-kD granule-associated protein (grp 60) from human LAK cells and from mouse cytolytic T cells. The NH2-terminal amino acid sequence of the polypeptide was found to be identical to calreticulin. Calreticulin is a calcium storage protein and carries a COOH-terminal KDEL sequence, known to act as a retention signal for proteins destined to the lumen of the endoplasmic reticulum. In CTLs, however, calreticulin colocalizes with the lytic perforin to the lysosome-like secretory granules, as confirmed by double label immunofluorescence confocal microscopy. Moreover, when the release of granule-associated proteins was triggered by stimulation of the T cell receptor complex, calreticulin was released along with granzymes A and D. Since perforin is activated and becomes lytic in the presence of calcium, we propose that the role of calreticulin is to prevent organelle autolysis due to the protein's calcium chelator capacity.
Mots-clé
Amino Acid Sequence Animals Calcium-Binding Proteins/analysis/*isolation & purification/secretion Calreticulin Cytoplasmic Granules/*chemistry Humans Mice Molecular Sequence Data Receptors, Antigen, T-Cell/physiology T-Lymphocytes, Cytotoxic/*chemistry
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 16:19
Dernière modification de la notice
08/05/2019 20:29
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