Prospero-related homeobox 1 gene (Prox1) is regulated by canonical Wnt signaling and has a stage-specific role in adult hippocampal neurogenesis.

Détails

Ressource 1Télécharger: PNAS-2011-Karalay-1013456108.pdf (1307.83 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_7513B4B8D875
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Prospero-related homeobox 1 gene (Prox1) is regulated by canonical Wnt signaling and has a stage-specific role in adult hippocampal neurogenesis.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Karalay O., Doberauer K., Vadodaria K.C., Knobloch M., Berti L., Miquelajauregui A., Schwark M., Jagasia R., Taketo M.M., Tarabykin V., Lie D.C., Jessberger S.
ISSN
1091-6490 (Electronic)
ISSN-L
0027-8424
Statut éditorial
Publié
Date de publication
05/04/2011
Peer-reviewed
Oui
Volume
108
Numéro
14
Pages
5807-5812
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Neural stem cells (NSCs) generate new granule cells throughout life in the mammalian hippocampus. Canonical Wnt signaling regulates the differentiation of NSCs towards the neuronal lineage. Here we identified the prospero-related homeodomain transcription factor Prox1 as a target of β-catenin-TCF/LEF signaling in vitro and in vivo. Prox1 overexpression enhanced neuronal differentiation whereas shRNA-mediated knockdown of Prox1 impaired the generation of neurons in vitro and within the hippocampal niche. In contrast, Prox1 was not required for survival of adult-generated granule cells after they had matured, suggesting a role for Prox1 in initial granule cell differentiation but not in the maintenance of mature granule cells. The data presented here characterize a molecular pathway from Wnt signaling to a transcriptional target leading to granule cell differentiation within the adult brain and identify a stage-specific function for Prox1 in the process of adult neurogenesis.
Mots-clé
Animals, Base Sequence, Blotting, Western, Cell Differentiation/physiology, Cells, Cultured, Chromatin Immunoprecipitation, DNA Primers/genetics, Hippocampus/cytology, Hippocampus/growth & development, Homeodomain Proteins/genetics, Homeodomain Proteins/metabolism, Immunohistochemistry, In Situ Hybridization, Luciferases, Mice, Molecular Sequence Data, Neural Stem Cells/metabolism, Neurogenesis/physiology, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction/physiology, Tumor Suppressor Proteins/genetics, Tumor Suppressor Proteins/metabolism, Wnt Proteins/metabolism
Pubmed
Web of science
Création de la notice
25/05/2018 9:36
Dernière modification de la notice
23/08/2018 6:26
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