Testosterone: a specific competitive antagonist of aldosterone in the toad bladder.

Details

Serval ID
serval:BIB_750E7F30C397
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Testosterone: a specific competitive antagonist of aldosterone in the toad bladder.
Journal
The American journal of physiology
Author(s)
Rossier B.C., Geering K., Gäggeler H.P., Claire M., Corvol P.
ISSN
0363-6127
Publication state
Published
Issued date
1980
Volume
239
Number
5
Pages
F433-9
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Abstract
Testosterone (100 nM to 40 microM) antagonized the effect of aldosterone (10 nM) on Na+ transport in the toad bladder measured in vitro as short-circuit current (SCC). Half-maximal inhibition occurred at an antagonist-agonist molar ratio of 150:1. The antagonist action of testosterone was reversed by addition of more aldosterone. The antagonism was specific in the sense that testosterone (20 microM) did not inhibit the response of the SCC to oxytocin (50 mU/ml). By itself, testosterone (up to 20 microM) had no agonist activity on base-line SCC. Finally, testosterone (500 nM to 20 microM) specifically displaced [3H]aldosterone (5 nm) from its cytoplasmic and nuclear binding sites in bladders incubated in vitro at 25 or 0 degrees C and labeled at steady state. There was a significant linear correlation between the effect of testosterone on the aldosterone-dependent SCC and its effect on [3H]aldosterone binding sites in the cytoplasm and in the nucleus. We conclude that 1) testosterone is a specific competitive antagonist of aldosterone, and 2) [3H]aldosterone nuclear and cytoplasmic binding sites could be mineralocorticoid receptors, mediating the action of aldosterone on Na+ transport.
Keywords
Aldosterone, Aldosterone Antagonists, Animals, Binding, Competitive, Biological Transport, Bufo marinus, Cell Nucleus, Cytoplasm, Female, Male, Receptors, Steroid, Sodium, Testosterone, Urinary Bladder
Pubmed
Web of science
Create date
24/01/2008 13:00
Last modification date
20/08/2019 14:32
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