Interactions between NPY and its receptor: assessment using ant-NPY antibodies

Détails

ID Serval
serval:BIB_7503C394A5F2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Interactions between NPY and its receptor: assessment using ant-NPY antibodies
Périodique
Regulatory Peptides
Auteur(s)
Grouzmann  E., Cressier  F., Walker  P., Hofbauer  K., Waeber  B., Brunner  H. R.
ISSN
0167-0115 (Print)
Statut éditorial
Publié
Date de publication
12/1994
Volume
54
Numéro
2-3
Pages
439-44
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec 15
Résumé
The present data show that monoclonal antibodies (NPY02, NPY03, NPY04, NPY05) directed against 4 distinct epitopes on NPY may have different actions on NPY binding and NPY-induced cellular responses. NPY02 and NPY05 recognize the 11-24 and 32-36 amidated form of NPY, respectively. These 2 antibodies block the binding of NPY to its receptor as well as the NPY-induced inhibition of cAMP accumulation caused in SK-N-MC cells by forskolin. NPY02 and NPY05 have also an inhibitory action on NPY-induced contraction of rabbit femoral arteries. NPY03 and NPY04 are directed against the 27-34 and 1-12 part of NPY, respectively. NPY03 and NPY04 inhibit the binding of NPY only at very high concentrations and have a weak effect on cAMP response to NPY. NPY02 and NPY05 might provide useful tools to study the effect of NPY in cellular systems and organ preparations.
Mots-clé
Amino Acid Sequence Antibodies, Monoclonal Cyclic AMP/metabolism Epitope Mapping Humans Immunologic Techniques Molecular Sequence Data Neuropeptide Y/*metabolism Radioligand Assay Receptors, Neuropeptide Y/*metabolism
Pubmed
Web of science
Création de la notice
25/01/2008 11:55
Dernière modification de la notice
03/03/2018 18:22
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