MYCN-related suppression of functional CD44 expression enhances tumorigenic properties of human neuroblastoma cells

Détails

ID Serval
serval:BIB_74F44AAB4CBD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
MYCN-related suppression of functional CD44 expression enhances tumorigenic properties of human neuroblastoma cells
Périodique
Experimental Cell Research
Auteur(s)
Gross  N., Balmas Bourloud  K., Brognara  C. B.
ISSN
0014-4827
Statut éditorial
Publié
Date de publication
11/2000
Peer-reviewed
Oui
Volume
260
Numéro
2
Pages
396-403
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov 1
Résumé
Highly malignant neuroblastoma tumors with MYCN amplification have been shown to downregulate the expression of the CD44 adhesion receptor. We have previously shown that MYCN amplified neuroblastoma cell lines either lack CD44 expression or express a nonfunctional, nonhyaluronic acid-binding CD44 receptor. By analysis of cells with manipulated expression of either CD44 or MYCN, we demonstrate that transfection of cells with a CD44 full-length cDNA construct produced a functional receptor in single copy MYCN cells and a nonfunctional CD44 receptor in MYCN amplified cells, similar to the CD44 receptor expressed by cells with enforced MYCN. Analysis of the in vivo growth properties of the transfectants revealed that the restoration of a functional CD44 receptor in nonamplified cells resulted in the suppression of in vivo cell growth, therefore linking the MYCN-related lack of hyaluronic acid-binding function of CD44 to the highly tumorigenic properties of a subset of neuroblastoma cells.
Mots-clé
Animals Antigens, CD44/genetics/metabolism Cell Adhesion Cytoplasm/metabolism Gene Expression Glycoproteins/genetics/metabolism Humans Hyaluronic Acid/metabolism Mice Mice, Inbred BALB C Mice, Nude Neuroblastoma/*physiopathology Proto-Oncogene Proteins c-myc/*genetics/physiology Rna Transfection Tumor Cells, Cultured
Pubmed
Web of science
Création de la notice
20/01/2008 16:55
Dernière modification de la notice
03/03/2018 18:22
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