Replicative phenotyping adds value to genotypic resistance testing in heavily pre-treated HIV-infected individuals--the Swiss HIV Cohort Study.

Détails

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Etat: Serval
Version: de l'auteur
ID Serval
serval:BIB_74E4E7A9A6E7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Replicative phenotyping adds value to genotypic resistance testing in heavily pre-treated HIV-infected individuals--the Swiss HIV Cohort Study.
Périodique
Journal of Translational Medicine
Auteur(s)
Fehr J., Glass T.R., Louvel S., Hamy F., Hirsch H.H., von Wyl V., Böni J., Yerly S., Bürgisser P., Cavassini M., Fux C.A., Hirschel B., Vernazza P., Martinetti G., Bernasconi E., Günthard H.F., Battegay M., Bucher H.C., Klimkait T.
Collaborateur(s)
Swiss HIV Cohort Study
Contributeur(s)
Battegay M., Bernasconi E., Böni J., Bucher HC., Bürgisser P., Calmy A., Cattacin S., Cavassini M., Dubs R., Egger M., Elzi L., Fehr J., Fischer M., Flepp M., Francioli P., Furrer H., Fux CA., Gorgievski M., Günthard HF., Hasse B., Hirsch HH., Hirschel B., Hösli I., Kahlert C., Kaiser L., Keiser O., Kind C., Klimkait T., Kovari H., Ledergerber B., Martinetti G., Müller N., Nadal D., Paccaud F., Pantaleo G., Rauch A., Regenass S., Rickenbach M., Rudin C., Schmid P., Schultze D., Schöni-Affolter F., Schüpbach J., Speck R., de Tejada BM. , Taffé P., Telenti A., Trkola A., Vernazza P., von Wyl V., Weber R., Yerly S.
ISSN
1479-5876 (Electronic)
ISSN-L
1479-5876
Statut éditorial
Publié
Date de publication
2011
Volume
9
Pages
14
Langue
anglais
Notes
Publication types: Evaluation Studies ; Journal Article ; Multicenter Study ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Résumé
BACKGROUND: Replicative phenotypic HIV resistance testing (rPRT) uses recombinant infectious virus to measure viral replication in the presence of antiretroviral drugs. Due to its high sensitivity of detection of viral minorities and its dissecting power for complex viral resistance patterns and mixed virus populations rPRT might help to improve HIV resistance diagnostics, particularly for patients with multiple drug failures. The aim was to investigate whether the addition of rPRT to genotypic resistance testing (GRT) compared to GRT alone is beneficial for obtaining a virological response in heavily pre-treated HIV-infected patients.
METHODS: Patients with resistance tests between 2002 and 2006 were followed within the Swiss HIV Cohort Study (SHCS). We assessed patients' virological success after their antiretroviral therapy was switched following resistance testing. Multilevel logistic regression models with SHCS centre as a random effect were used to investigate the association between the type of resistance test and virological response (HIV-1 RNA <50 copies/mL or ≥1.5 log reduction).
RESULTS: Of 1158 individuals with resistance tests 221 with GRT+rPRT and 937 with GRT were eligible for analysis. Overall virological response rates were 85.1% for GRT+rPRT and 81.4% for GRT. In the subgroup of patients with >2 previous failures, the odds ratio (OR) for virological response of GRT+rPRT compared to GRT was 1.45 (95% CI 1.00-2.09). Multivariate analyses indicate a significant improvement with GRT+rPRT compared to GRT alone (OR 1.68, 95% CI 1.31-2.15).
CONCLUSIONS: In heavily pre-treated patients rPRT-based resistance information adds benefit, contributing to a higher rate of treatment success.
Mots-clé
Adult, Anti-HIV Agents/pharmacology, Anti-HIV Agents/therapeutic use, Cohort Studies, Dose-Response Relationship, Drug, Drug Resistance, Viral/physiology, Female, Genotype, HIV Infections/diagnosis, HIV Infections/drug therapy, HIV-1/physiology, Humans, Male, Microbial Sensitivity Tests/methods, Middle Aged, Phenotype, Predictive Value of Tests, Prognosis, Switzerland, Virus Replication/drug effects, Virus Replication/physiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
22/03/2011 12:04
Dernière modification de la notice
08/05/2019 20:29
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