Article: article from journal or magazin.
Identification and characterization of an anti-tyrosine kinase factor in cystic gliomas
Journal Article --- Old month value: Jan 25
In view of the frequent activation of the epidermal growth factor receptor (EGF-R) in gliomas and autocrine hypothesis, we searched for 'EGF-like' factor(s) in cystic fluids (CFs) associated with gliomas. Membranes of A431 cells, which overexpress EGF-R, were used to explore such activity in 20 CFs. In all cases CFs induced inhibition of EGF-R phosphorylation. Biochemical analysis revealed an anti-tyrosine kinase activity which was identified as a 18 kDa proteic factor. Effectiveness at high dilution and anti-proliferative effect on living cells in culture suggest that this factor may be involved in the negative regulation of glial oncogenesis.
Astrocytoma/*chemistry Brain Neoplasms/*chemistry Chromatography, High Pressure Liquid Electrophoresis, Polyacrylamide Gel Humans Phosphorylation Protein-Tyrosine Kinases/*antagonists & inhibitors Receptor, Epidermal Growth Factor/metabolism Tumor Cells, Cultured
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