Molecular basis for the defective expression of the mouse Ew17 beta gene.

Détails

ID Serval
serval:BIB_74A68864864E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Molecular basis for the defective expression of the mouse Ew17 beta gene.
Périodique
Journal of Immunology
Auteur(s)
Vu T.H., Tacchini-Cottier F.M., Day C.E., Begovich A.B., Jones P.P.
ISSN
0022-1767 (Print)
ISSN-L
0022-1767
Statut éditorial
Publié
Date de publication
1988
Volume
141
Numéro
10
Pages
3654-3661
Langue
anglais
Résumé
Four of the eleven independent H-2 haplotypes of inbred mouse strains and approximately 15% of wild mouse chromosomes 17 fail to express the E alpha E beta class II histocompatibility (Ia) Ag. These E- haplotypes are defective in the expression of the E alpha and/or the E beta chain. None of the E beta defects has previously been described at the molecular level. In this study, we report the molecular basis for the defective expression of the E beta gene from the w17 haplotype of the H-2 congenic strain B10.CAS2, derived from wild Mus musculus castaneus. Comparison of the Ew17 beta genomic sequence to those of the functional Eb beta and Ed beta genes reveals a single base insertion in the RNA donor splice site of the first intron. By DNA shuffling, we have corrected the single base mutation, and we show by FACS analysis and 2-D PAGE of immunoprecipitates that the corrected Ew17 beta is expressed in L cells when co-transfected with an Ed alpha gene. Conversely, an Eb beta gene construct containing the mutant RNA splice site from Ew17 beta is not expressed. We conclude that the single base insertion in the first RNA splice donor site is the sole molecular defect in the Ew17 beta gene.
Mots-clé
Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, Genes, H-2 Antigens/genetics, Haplotypes, Histocompatibility Antigens Class II/deficiency, Histocompatibility Antigens Class II/genetics, Mice, Molecular Sequence Data, Mutation, RNA/deficiency, RNA/genetics, RNA Splicing, Transfection
Pubmed
Web of science
Création de la notice
24/01/2008 16:09
Dernière modification de la notice
20/08/2019 15:32
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