Molecular beacon sequence analysis for detecting drug resistance in Mycobacterium tuberculosis

Details

Serval ID
serval:BIB_7485BF2BBB9E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Molecular beacon sequence analysis for detecting drug resistance in Mycobacterium tuberculosis
Journal
Nature Biotechnology
Author(s)
Piatek  A. S., Tyagi  S., Pol  A. C., Telenti  A., Miller  L. P., Kramer  F. R., Alland  D.
ISSN
1087-0156 (Print)
Publication state
Published
Issued date
04/1998
Volume
16
Number
4
Pages
359-63
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Apr
Abstract
We developed a new approach to DNA sequence analysis that uses fluorogenic reporter molecules--molecular beacons--and demonstrated their ability to discriminate alleles in real-time PCR assays of genomic DNA. A set of overlapping molecular beacons was used to analyze an 81-bp region of the Mycobacterium tuberculosis rpoB gene for mutations that confer resistance to the antibiotic rifampin. In a blinded study of 52 rifampin-resistant and 23 rifampin-susceptible clinical isolates, this method correctly detected mutations in all of the resistant strains and in none of the susceptible strains. The assay was carried out entirely in sealed PCR tubes and was simple to perform and interpret. This approach can be used to analyze any DNA sequence of moderate length with single base pair accuracy.
Keywords
Antibiotics, Antitubercular/pharmacology Drug Resistance, Microbial/*genetics Microbial Sensitivity Tests Mycobacterium tuberculosis/*drug effects/genetics Plant Proteins/genetics *Point Mutation Polymerase Chain Reaction Rifampin/pharmacology
Pubmed
Web of science
Create date
25/01/2008 14:45
Last modification date
20/08/2019 14:32
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