Human macrophages respond to LPS in a serum-independent, CD14-dependent manner

Détails

ID Serval
serval:BIB_746E82E98440
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Human macrophages respond to LPS in a serum-independent, CD14-dependent manner
Périodique
Immunology Letters
Auteur(s)
Jungi  T. W., Brcic  M., Eperon  S.
ISSN
0165-2478
Statut éditorial
Publié
Date de publication
12/1996
Peer-reviewed
Oui
Volume
54
Numéro
1
Pages
37-43
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec 1
Résumé
Two crucial mediators of monocyte activation by lipopolysaccharide (LPS) are the acute phase plasma factor, lipopolysaccharide binding protein (LBP) and cell-surface-expressed CD14. Whether macrophage (M phi) recognized and respond to LPS in a similar manner is unknown. Here we show that human monocyte-derived M phi respond to LPS by tumor necrosis factor-alpha release and procoagulant activity upregulation by a similar dose response curve in the presence or absence of serum, suggesting that humoral factors such as LBP are relatively unimportant in the activation of M phi. Both serum-dependent and serum-independent activation of M phi by LPS require cellular CD14, as evidence by blocking studies with CD14-specific antibodies. Clones from the monocytoid cell line Mono Mac-6 selected for high LPS sensitivity displayed similar properties. When washed free of serum and cultured in the presence of calcitriol, they responded to LPS in a similar manner, regardless of the presence or absence of serum, and this response was inhibited by anti-CD14. It is hypothesized that during their differentiation. M phi acquire a functional substitute for the serum factor LBP, thereby being able to recognize low LPS concentrations in a milieu low in LBP concentration. It will be of interest to determine whether this is a high-affinity LBP receptor, LBP itself, or another cell surface constituent.
Mots-clé
Antibodies/immunology Antigens, CD14/*immunology Blood Cell Line Humans Lipopolysaccharides/*immunology Macrophages/drug effects/*immunology Mitogens/*immunology Tumor Necrosis Factor-alpha/biosynthesis
Pubmed
Web of science
Création de la notice
17/01/2008 14:27
Dernière modification de la notice
03/03/2018 18:21
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