The fragile X mental retardation protein-RNP granules show an mGluR-dependent localization in the post-synaptic spines.

Détails

ID Serval
serval:BIB_745199214325
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The fragile X mental retardation protein-RNP granules show an mGluR-dependent localization in the post-synaptic spines.
Périodique
Molecular and cellular neurosciences
Auteur(s)
Ferrari F., Mercaldo V., Piccoli G., Sala C., Cannata S., Achsel T., Bagni C.
ISSN
1044-7431 (Print)
ISSN-L
1044-7431
Statut éditorial
Publié
Date de publication
03/2007
Volume
34
Numéro
3
Pages
343-354
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The localization of RNA/mRNA in dendrites plays a role in both local and temporal regulation of protein synthesis, which is required for certain forms of synaptic plasticity. A key molecule in these processes is the fragile X mental retardation protein (FMRP). Using in situ hybridization coupled to immunofluorescence confocal microscopy, we find that the FMRP-RNP particle contains alphaCaMKII and BC1 RNAs as well as Staufen and CPEB proteins. Furthermore, following mGluR activation, the FMRP-mRNP complex moves into spines as shown by co-localization with the PSD-95 and Shank proteins. This study shows, for the first time, that the translationally inactive FMRP-mRNP complex relocates into neuronal spines after stimulation and that de novo protein synthesis mainly contributes to the pool of FMRP at synapses.

Mots-clé
Animals, Brain/cytology, Cells, Cultured, Cercopithecus aethiops, Cricetinae, Dendritic Spines/drug effects, Dendritic Spines/metabolism, Embryo, Mammalian, Fluorescent Antibody Technique/methods, Fragile X Mental Retardation Protein/metabolism, Gene Expression Regulation, Developmental/drug effects, Gene Expression Regulation, Developmental/physiology, Guanylate Kinases, In Situ Hybridization, Intracellular Signaling Peptides and Proteins/metabolism, Membrane Proteins/metabolism, Methoxyhydroxyphenylglycol/analogs & derivatives, Methoxyhydroxyphenylglycol/pharmacology, Mice, Microtubule-Associated Proteins/metabolism, Nerve Tissue Proteins/metabolism, Neurons/cytology, RNA-Binding Proteins/metabolism, Receptors, Metabotropic Glutamate/antagonists & inhibitors, Receptors, Metabotropic Glutamate/physiology, Time Factors, Transfection, tau Proteins/metabolism
Pubmed
Création de la notice
06/03/2017 18:23
Dernière modification de la notice
03/03/2018 18:21
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