Rab17 localizes to recycling endosomes and regulates receptor-mediated transcytosis in epithelial cells.

Détails

ID Serval
serval:BIB_7446
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Rab17 localizes to recycling endosomes and regulates receptor-mediated transcytosis in epithelial cells.
Périodique
Journal of Biological Chemistry
Auteur(s)
Hunziker W., Peters P.J.
ISSN
0021-9258
Statut éditorial
Publié
Date de publication
1998
Volume
273
Numéro
25
Pages
15734-15741
Langue
anglais
Résumé
The small GTPase Rab17 is restricted to epithelial cells and its expression is induced during cell polarization. This observation has led to the suggestion that the protein may function in transcytosis, a pathway connecting the apical and basolateral endocytic systems. To analyze whether Rab17 plays a role in transcellular transport, we generated Madin-Darby canine kidney (MDCK) cell lines stably coexpressing wild-type or mutant Rab17 and the transcytotic polymeric immunoglobulin receptor (pIgR). Rab17 expressed in MDCK cells was found on small vesicles and tubules in the apical region of the cells. A significant fraction of the Rab17-positive structures was accessible to dimeric IgA internalized from the apical or basolateral cell surface via the pIgR. Furthermore, basolateral to apical transcytosis of dimeric IgA was impaired in MDCK cells overexpressing Rab17. Our data provides morphological and biochemical evidence for a role of Rab17 in the regulation of transcellular traffic through apical recycling endosomes in epithelial cells.
Mots-clé
Animals, Biological Transport, Cell Line, Cell Polarity, Dogs, Endosomes/metabolism, Epithelial Cells/metabolism, GTP Phosphohydrolases/metabolism, Mice, Microscopy, Fluorescence, Receptors, Polymeric Immunoglobulin/metabolism, rab GTP-Binding Proteins
Pubmed
Web of science
Open Access
Oui
Création de la notice
19/11/2007 13:45
Dernière modification de la notice
08/05/2019 20:27
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