Production of Fusaric Acid by Fusarium spp. in Pure Culture and in Solid Medium Co-Cultures.

Détails

Ressource 1Télécharger: BIB_743ECA11ABA1.P001.pdf (1991.09 [Ko])
Etat: Serval
Version: de l'auteur
ID Serval
serval:BIB_743ECA11ABA1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Production of Fusaric Acid by Fusarium spp. in Pure Culture and in Solid Medium Co-Cultures.
Périodique
Molecules
Auteur(s)
Bohni N., Hofstetter V., Gindro K., Buyck B., Schumpp O., Bertrand S., Monod M., Wolfender J.L.
ISSN
1420-3049 (Electronic)
ISSN-L
1420-3049
Statut éditorial
Publié
Date de publication
2016
Peer-reviewed
Oui
Volume
21
Numéro
3
Pages
1-16
Langue
anglais
Résumé
The ability of fungi isolated from nails of patients suffering from onychomycosis to induce de novo production of bioactive compounds in co-culture was examined. Comparison between the metabolite profiles produced by Sarocladium strictum, by Fusarium oxysporum, and by these two species in co-culture revealed de novo induction of fusaric acid based on HRMS. Structure confirmation of this toxin, using sensitive microflow NMR, required only three 9-cm Petri dishes of fungal culture. A targeted metabolomics study based on UHPLC-HRMS confirmed that the production of fusaric acid was strain-dependent. Furthermore, the detected toxin levels suggested that onychomycosis-associated fungal strains of the F. oxysporum and F. fujikuroi species complexes are much more frequently producing fusaric acid, and in higher amount, than strains of the F. solani species complex. Fusarium strains producing no significant amounts of this compound in pure culture, were shown to de novo produce that compound when grown in co-culture. The role of fusaric acid in fungal virulence and defense is discussed.
Pubmed
Web of science
Open Access
Oui
Création de la notice
10/04/2016 14:50
Dernière modification de la notice
08/05/2019 20:27
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