Apical and basolateral membrane conductances in the TBM cell line

Détails

ID Serval
serval:BIB_7404A0EF91D8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Apical and basolateral membrane conductances in the TBM cell line
Périodique
American Journal of Physiology
Auteur(s)
Horisberger  J. D.
ISSN
0002-9513 (Print)
Statut éditorial
Publié
Date de publication
06/1991
Volume
260
Numéro
6 Pt 1
Pages
C1172-81
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Résumé
Cultured cell lines present several advantages over whole organ or ex vivo isolated epithelium for the physiological and biochemical study of epithelial transport. We have developed a new technique allowing for simultaneous intracellular and transepithelial electrophysiological measurements in the epithelium formed by a cultured cell line grown on thin collagen membranes. This technique was applied to the TBM 18/23 (toad bladder origin) cell line. The transepithelial and basolateral membrane potentials were -30 +/- 11 and -72 +/- 8 (SD) mV (n = 36), respectively. With the use of the effect of amiloride, which partially blocked the apical membrane conductance, and circuit analysis, the apical and basolateral membrane conductances were estimated to 0.7 +/- 0.1 and 2.8 +/- 0.4 mS/cm2, respectively. A sodium-selective conductive pathway was demonstrated in the apical membrane, and a barium-sensitive K(+)-selective conductance was shown to be present in the basolateral membrane. The basolateral membrane conductance was not modified by sudden inhibition of sodium transport by amiloride, but it was significantly reduced after a long-term decrease of Na+ transport. The cultured TBM cell line appears to be a convenient model to investigate the regulation of membrane ionic conductances in tight epithelia.
Mots-clé
Amiloride/pharmacology Animals Bufo marinus Cell Line Cell Membrane/drug effects/*physiology/ultrastructure Collagen Culture Techniques/instrumentation/methods Electric Conductivity Electrophysiology/instrumentation/methods Epithelium/physiology Kinetics Mathematics Membrane Potentials/drug effects Microscopy, Electron Urinary Bladder/physiology
Pubmed
Web of science
Création de la notice
24/01/2008 13:37
Dernière modification de la notice
03/03/2018 18:20
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