Activation of the U2 snRNA promoter by the octamer motif defines a new class of RNA polymerase II enhancer elements.

Détails

ID Serval
serval:BIB_73F32C8E72E4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Activation of the U2 snRNA promoter by the octamer motif defines a new class of RNA polymerase II enhancer elements.
Périodique
Genes and Development
Auteur(s)
Tanaka M., Grossniklaus U., Herr W., Hernandez N.
ISSN
0890-9369[print], 0890-9369[linking]
Statut éditorial
Publié
Date de publication
12/1988
Volume
2
Numéro
12B
Pages
1764-1778
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
The recent discovery that the activation domains of transcriptional activators (e.g., GAL4) from a number of species are interchangeable has led to the concept of a general mechanism for activation of RNA polymerase II genes. We have examined the different activities of the SV40 octamer motif ATGCAAAG in B cells and in HeLa cells in the context of either the beta-globin promoter, a TATA-box-containing mRNA promoter, or the U2 snRNA promoter, which contains a snRNA-specific proximal element. In the context of the beta-globin promoter, the octamer motif is a B-cell-specific enhancer element, whereas it is a ubiquitous enhancer element for the U2 snRNA promoter. The U2 promoter is unique in that it is not activated by enhancer elements that activate the beta-globin promoter, and a hybrid U2 promoter containing the upstream activating sequence UASG is not stimulated by a yeast GAL4 trans-activator. Together, these observations suggest that in the context of the U2 promoter, the octamer motif defines a new class of RNA polymerase II enhancer elements, which bind transcription factors that trans-activate gene expression by a different mechanism than the general mechanism mentioned above. These results are discussed in light of the possibility that the ubiquitous octamer binding protein Oct-1 and the B-cell-specific octamer binding protein Oct-2 are involved in the activation of the U2 and beta-globin promoters, respectively.
Mots-clé
B-Lymphocytes, DNA-Binding Proteins, Enhancer Elements, Genetic, Fungal Proteins/genetics, Globins/genetics, Hela Cells, Humans, Models, Genetic, Plasmids, Promoter Regions, Genetic, RNA Polymerase II/genetics, RNA, Small Nuclear/genetics, Saccharomyces cerevisiae Proteins, Simian virus 40/genetics, Simplexvirus/genetics, Transcription Factors/genetics, Transfection, Viral Proteins/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 16:36
Dernière modification de la notice
20/08/2019 15:31
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