Lysosomal enzymes in preterm infants with bronchopulmonary dysplasia: a potential diagnostic marker

Détails

ID Serval
serval:BIB_7399F9F88B51
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Lysosomal enzymes in preterm infants with bronchopulmonary dysplasia: a potential diagnostic marker
Périodique
Clinica Chimica Acta
Auteur(s)
Goi  G., Bairati  C., Massaccesi  L., Lombardo  A., Bonafe  L., Zanardo  V., Burlina  A.
ISSN
0009-8981
Statut éditorial
Publié
Date de publication
11/1998
Peer-reviewed
Oui
Volume
278
Numéro
1
Pages
23-34
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Résumé
Some lysosomal glycohydrolases (N-acetyl-beta-D-glucosaminidase and their major isoenzymes, beta-D-glucuronidase, alpha-D-galactosidase, beta-D-galactosidase and alpha-D-glucosidase) were investigated in the plasma of 36 preterm infants with respiratory distress, 11 of whom developed bronchopulmonary dysplasia (BPD), in order to evaluate the role of the lysosomal apparatus in the disease. Enzyme activity was assayed fluorimetrically; the major N-acetyl-beta-D-glucosaminidase (NAG) isoenzymes were separated using a routine chromatofocusing procedure; the diagnostic efficiency was evaluated by Bayes theorem. The mean levels of almost all glycohydrolases considered were significantly higher in BPD than in non-BPD infants. Among NAG major isoenzymes, an increase was found only in form A. No variation was evident in the plasma levels of glycohydrolases during dexamethasone therapy. Data from a retrospective analysis performed in all preterms considered, show that alpha-D-galactosidase and beta-D-galactosidase differentiate a posteriori BPD and non-BPD subjects. These enzymes, after a priori verification of their diagnostic potential in preterm infants at risk of BPD development, could acquire an important predictive value.
Mots-clé
Biological Markers/*blood Bronchopulmonary Dysplasia/drug therapy/*enzymology Dexamethasone/therapeutic use Glycoside Hydrolases/*blood Humans Infant, Newborn Infant, Premature/*blood Lysosomes/*enzymology
Pubmed
Web of science
Création de la notice
21/01/2008 13:50
Dernière modification de la notice
03/03/2018 18:19
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