The presence of a CD4+ CD25high CD45RO+ CD127high T-cell population correlates with the clinical status of kidney transplant recipients
Details
Serval ID
serval:BIB_7383265DF5F4
Type
Inproceedings: an article in a conference proceedings.
Publication sub-type
Poster: Summary – with images – on one page of the results of a researche project. The summaries of the poster must be entered in "Abstract" and not "Poster".
Collection
Publications
Institution
Title
The presence of a CD4+ CD25high CD45RO+ CD127high T-cell population correlates with the clinical status of kidney transplant recipients
Title of the conference
Annual Joint Meeting of the Swiss Societies for Pneumology, Paediatric Pneumology, Allergology and Immunology, Thoracic Surgery
Address
Fribourg, Switzerland, April 17-18, 2008
ISBN
1424-7860
Publication state
Published
Issued date
2008
Peer-reviewed
Oui
Volume
138
Series
Swiss Medical Weekly
Pages
57S
Language
english
Notes
Meeting Abstract
Abstract
Background: Recent data have suggested that a population of CD4+
CD25high T cells, phenotypically characterized by the expression of
CD45RO and CD127, is significantly expanded in stable liver and
kidney transplant recipients and represents alloreactive T cells. We
analyzed this putative new alloreactive cellular marker in various
groups of kidney transplant recipients.
Patients and methods: Flow cytometry was used to analyze the
expression of CD25, CD45RO and CD127 on peripheral CD4+ T cells.
Of 73 kidney recipients, 59 had a stable graft function under standard
immunosuppressive therapy (IS), 5 had biopsy-proven chronic
humoral rejection (CHR), 8 were stable under minimal IS and one was
an operationally "tolerant" patient who had discontinued IS for more
than 3 years. Sixty-six healthy subjects (HS) were studied as controls.
Results: Overall, the alloreactive T cell population was found to be
significantly increased in the 73 kidney recipients (mean ± SE: 15.03 ±
1.04% of CD4+ CD25high T cells) compared to HS (5.93 ± 0.39%)
(p <0.001). In the 5 patients with CHR, this population was highly
expanded (31.33 ± 4.16%), whereas it was comparable to HS in the
8 stable recipients receiving minimal IS (6.12 ± 0.86%), in 4 patients
who had been switched to sirolimus (4.21 ± 0.53%) as well as in
the unique "tolerant" recipient (4.69%). Intermediate levels (15.84 ±
0.93%) were found in the 55 recipients with stable graft function
on standard CNI-based IS. Regulatory T cells, defined as CD4+
CD25high FoxP3+ CD127low, were found to be significantly reduced
in all recipients except in those with minimal or no IS, and this
reduction was particularly striking in recipients with CHR.
Conclusion: After kidney transplantation, an alloreactive T cell
population was found to be significantly expanded and it correlates
with the clinical status of the recipients. Interestingly, in stable
patients with minimal (or no) IS as well as in patients on sirolimus,
alloreactive T cells were comparable the healthy controls. Measuring
circulating CD4+ CD25high CD45RO+ CD127high T cells may
become a useful monitoring tool after transplantation.
CD25high T cells, phenotypically characterized by the expression of
CD45RO and CD127, is significantly expanded in stable liver and
kidney transplant recipients and represents alloreactive T cells. We
analyzed this putative new alloreactive cellular marker in various
groups of kidney transplant recipients.
Patients and methods: Flow cytometry was used to analyze the
expression of CD25, CD45RO and CD127 on peripheral CD4+ T cells.
Of 73 kidney recipients, 59 had a stable graft function under standard
immunosuppressive therapy (IS), 5 had biopsy-proven chronic
humoral rejection (CHR), 8 were stable under minimal IS and one was
an operationally "tolerant" patient who had discontinued IS for more
than 3 years. Sixty-six healthy subjects (HS) were studied as controls.
Results: Overall, the alloreactive T cell population was found to be
significantly increased in the 73 kidney recipients (mean ± SE: 15.03 ±
1.04% of CD4+ CD25high T cells) compared to HS (5.93 ± 0.39%)
(p <0.001). In the 5 patients with CHR, this population was highly
expanded (31.33 ± 4.16%), whereas it was comparable to HS in the
8 stable recipients receiving minimal IS (6.12 ± 0.86%), in 4 patients
who had been switched to sirolimus (4.21 ± 0.53%) as well as in
the unique "tolerant" recipient (4.69%). Intermediate levels (15.84 ±
0.93%) were found in the 55 recipients with stable graft function
on standard CNI-based IS. Regulatory T cells, defined as CD4+
CD25high FoxP3+ CD127low, were found to be significantly reduced
in all recipients except in those with minimal or no IS, and this
reduction was particularly striking in recipients with CHR.
Conclusion: After kidney transplantation, an alloreactive T cell
population was found to be significantly expanded and it correlates
with the clinical status of the recipients. Interestingly, in stable
patients with minimal (or no) IS as well as in patients on sirolimus,
alloreactive T cells were comparable the healthy controls. Measuring
circulating CD4+ CD25high CD45RO+ CD127high T cells may
become a useful monitoring tool after transplantation.
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Create date
13/10/2009 14:28
Last modification date
20/08/2019 15:31
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