Maintaining dendritic cell viability in culture.

Détails

ID Serval
serval:BIB_7380D813A5B2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Maintaining dendritic cell viability in culture.
Périodique
Molecular Immunology
Auteur(s)
Vremec D., Hansen J., Strasser A., Acha-Orbea H., Zhan Y., O'Keeffe M., Shortman K.
ISSN
1872-9142 (Electronic)
ISSN-L
0161-5890
Statut éditorial
Publié
Date de publication
2015
Volume
63
Numéro
2
Pages
264-267
Langue
anglais
Résumé
When mouse dendritic cells (DCs) are isolated from tissues, purified and placed in a nutritive culture they die more rapidly than would be expected from their normal turnover in vivo. This can distort culture assays of DC function. We therefore tested several approaches to prolonging DC survival in culture. Of several cytokines tested granulocyte-macrophage colony stimulating factor was most effective at preserving the viability of conventional DCs (cDCs) but was ineffective for plasmacytoid DCs (pDCs). Surprisingly, Fms-like tyrosine kinase 3 ligand, crucial for DC development, produced only a marginal improvement in DC survival in culture, and interleukin-3, reported to prevent apoptosis of human pDCs, produced only a minor improvement in survival of mouse DCs. Genetic manipulation of cell death pathways was also tested, to avoid activation effects exerted by cytokine signalling. The isolation of DCs from mice overexpressing Bcl-2 was especially effective in maintaining pDC viability but gave a lesser improvement in cDC viability. DCs isolated from Bim(-/-)Noxa(-/-) mice also showed improved culture survival, but in this case with pDCs showing the least improvement.
Pubmed
Web of science
Création de la notice
17/01/2015 12:02
Dernière modification de la notice
03/03/2018 18:19
Données d'usage