Cloning and characterization of cDNAs that code for Na(+)-channel-blocking toxins of the scorpion Centruroides noxius Hoffmann.

Détails

ID Serval
serval:BIB_7379E4C28ACC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Cloning and characterization of cDNAs that code for Na(+)-channel-blocking toxins of the scorpion Centruroides noxius Hoffmann.
Périodique
Gene
Auteur(s)
Becerril B., Vázquez A., García C., Corona M., Bolivar F., Possani L.D.
ISSN
0378-1119
Statut éditorial
Publié
Date de publication
1993
Peer-reviewed
Oui
Volume
128
Numéro
2
Pages
165-171
Langue
anglais
Résumé
With the purpose of studying the organization and characteristics of the genes that code for toxins present in the venom of the Mexican scorpion, Centruroides noxius Hoffmann (CnH), we prepared a lambda gt11 cDNA library from the venom glands. Using specific oligodeoxyribonucleotides (oligos) designed according to known amino acid (aa) sequences of CnH toxins (STox), we detected several positive clones, determined their nucleotide (nt) sequences and deduced their aa sequences. A comparative analysis of these sequences with previously reported STox revealed that CnH cDNAs code for a family of very similar STox. The cDNA coding for a known STox, II-10, was cloned. Additionally, three other complete (new) nt sequences were obtained for cDNAs encoding peptides similar to STox 1 from CnH or variants 2 and 3 from Centruroides sculpturatus Ewing. Southern blot genomic DNA analysis showed a minimum size of approximately 600 bp as EcoRI fragments for elements of this family. PCR amplifications of CnH genomic DNA and hybridization of PCR products with specific probes indicated that the genomic structural regions that code for these genes do not contain introns, or at least not large introns.
Mots-clé
Amino Acid Sequence, Base Sequence, Blotting, Southern, Cloning, Molecular, DNA, Single-Stranded/analysis, Genes, Molecular Sequence Data, Multigene Family, Polymerase Chain Reaction, Scorpion Venoms/genetics, Scorpion Venoms/toxicity, Sequence Analysis, DNA, Sodium Channels/drug effects, Toxins, Biological/genetics
Pubmed
Web of science
Création de la notice
10/03/2008 13:00
Dernière modification de la notice
03/03/2018 18:19
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