Enhanced sensitivity detection of protein immobilization by fluorescent interference on oxidized silicon.

Détails

ID Serval
serval:BIB_736547BDF053
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Enhanced sensitivity detection of protein immobilization by fluorescent interference on oxidized silicon.
Périodique
Biosensors and Bioelectronics
Auteur(s)
Volle J.N., Chambon G., Sayah A., Reymond C., Fasel N., Gijs M.A.
ISSN
0956-5663 (Print)
ISSN-L
0956-5663
Statut éditorial
Publié
Date de publication
2003
Volume
19
Numéro
5
Pages
457-464
Langue
anglais
Résumé
We present a silicon chip-based approach for the enhanced sensitivity detection of surface-immobilized fluorescent molecules. Green fluorescent protein (GFP) is bound to the silicon substrate by a disuccinimidyl terephtalate-aminosilane immobilization procedure. The immobilized organic layers are characterized by surface analysis techniques, like ellipsometry, atomic force microscopy (AFM) and X-ray induced photoelectron spectroscopy. We obtain a 20-fold enhancement of the fluorescent signal, using constructive interference effects in a fused silica dielectric layer, deposited before immobilization onto the silicon. Our method opens perspectives to increase by an order of magnitude the fluorescent response of surface immobilized DNA- or protein-based layers for a variety of biosensor applications.
Mots-clé
Adsorption, Biosensing Techniques/instrumentation, Biosensing Techniques/methods, Green Fluorescent Proteins, Luminescent Proteins/analysis, Luminescent Proteins/chemistry, Membranes, Artificial, Oxidation-Reduction, Proteins/analysis, Proteins/chemistry, Reproducibility of Results, Sensitivity and Specificity, Silanes/chemistry, Silicon/chemistry, Spectrometry, Fluorescence/methods, Surface Properties
Pubmed
Web of science
Création de la notice
24/01/2008 16:02
Dernière modification de la notice
03/03/2018 18:19
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