Article: article from journal or magazin.
Enhanced sensitivity detection of protein immobilization by fluorescent interference on oxidized silicon.
Biosensors and Bioelectronics
We present a silicon chip-based approach for the enhanced sensitivity detection of surface-immobilized fluorescent molecules. Green fluorescent protein (GFP) is bound to the silicon substrate by a disuccinimidyl terephtalate-aminosilane immobilization procedure. The immobilized organic layers are characterized by surface analysis techniques, like ellipsometry, atomic force microscopy (AFM) and X-ray induced photoelectron spectroscopy. We obtain a 20-fold enhancement of the fluorescent signal, using constructive interference effects in a fused silica dielectric layer, deposited before immobilization onto the silicon. Our method opens perspectives to increase by an order of magnitude the fluorescent response of surface immobilized DNA- or protein-based layers for a variety of biosensor applications.
Adsorption, Biosensing Techniques/instrumentation, Biosensing Techniques/methods, Green Fluorescent Proteins, Luminescent Proteins/analysis, Luminescent Proteins/chemistry, Membranes, Artificial, Oxidation-Reduction, Proteins/analysis, Proteins/chemistry, Reproducibility of Results, Sensitivity and Specificity, Silanes/chemistry, Silicon/chemistry, Spectrometry, Fluorescence/methods, Surface Properties
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