Enhanced sensitivity detection of protein immobilization by fluorescent interference on oxidized silicon.

Details

Serval ID
serval:BIB_736547BDF053
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Enhanced sensitivity detection of protein immobilization by fluorescent interference on oxidized silicon.
Journal
Biosensors and Bioelectronics
Author(s)
Volle J.N., Chambon G., Sayah A., Reymond C., Fasel N., Gijs M.A.
ISSN
0956-5663 (Print)
ISSN-L
0956-5663
Publication state
Published
Issued date
2003
Volume
19
Number
5
Pages
457-464
Language
english
Abstract
We present a silicon chip-based approach for the enhanced sensitivity detection of surface-immobilized fluorescent molecules. Green fluorescent protein (GFP) is bound to the silicon substrate by a disuccinimidyl terephtalate-aminosilane immobilization procedure. The immobilized organic layers are characterized by surface analysis techniques, like ellipsometry, atomic force microscopy (AFM) and X-ray induced photoelectron spectroscopy. We obtain a 20-fold enhancement of the fluorescent signal, using constructive interference effects in a fused silica dielectric layer, deposited before immobilization onto the silicon. Our method opens perspectives to increase by an order of magnitude the fluorescent response of surface immobilized DNA- or protein-based layers for a variety of biosensor applications.
Keywords
Adsorption, Biosensing Techniques/instrumentation, Biosensing Techniques/methods, Green Fluorescent Proteins, Luminescent Proteins/analysis, Luminescent Proteins/chemistry, Membranes, Artificial, Oxidation-Reduction, Proteins/analysis, Proteins/chemistry, Reproducibility of Results, Sensitivity and Specificity, Silanes/chemistry, Silicon/chemistry, Spectrometry, Fluorescence/methods, Surface Properties
Pubmed
Web of science
Create date
24/01/2008 15:02
Last modification date
20/08/2019 14:31
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