Rapid induction of cytolytic T cells via CD28 stimulation for cellular immunotherapy

Détails

ID Serval
serval:BIB_73485D47A8C2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Rapid induction of cytolytic T cells via CD28 stimulation for cellular immunotherapy
Périodique
Therapeutic Immunology
Auteur(s)
Blum  S., Milesi  R., Tratkiewicz  J., Olive  D., Gallati  H., Cerottini  J. C., von Fliedner  V.
ISSN
0967-0149 (Print)
Statut éditorial
Publié
Date de publication
06/1994
Volume
1
Numéro
3
Pages
143-52
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Résumé
One approach to adoptive cancer immunotherapy is based on the use of bispecific monoclonal antibodies (mAb) capable to redirect ex vivo generated cytolytic T lymphocytes (CTL) onto tumour cells. The efficiency of the CD28 T-cell activation pathway to induce CD3-dependent cytolytic activity was investigated while avoiding modulation of the TCR/CD3 complex needed for targeting by bispecific mAb. When used e.g. in conjunction with anti-CD2 antibodies or diacylglycerol derivatives, the in vitro stimulation of T cells with anti-CD28 mAb resulted within 36 h in high levels of CD3-dependent cytolysis (tested on a FcR+ target in the presence of anti-CD3 mAb) and sustained lymphokine production, such as TNF alpha, IFN gamma and IL-2, which may affect tumour growth when delivered locally by the transferred T cells. Rapid activation may reduce costly in vitro procedures, preserve homing capacities of retransfused T cells, and thus facilitate implementation of clinical trials based on the use of bispecific antibodies.
Mots-clé
Antibodies, Bispecific/therapeutic use Antibodies, Monoclonal/immunology Antigens, CD28/*immunology Antigens, CD3/immunology Cells, Cultured Cytokines/genetics Cytotoxicity, Immunologic Diglycerides/pharmacology Enzyme Activation/drug effects Gene Expression Humans Immunotherapy, Adoptive/methods *Lymphocyte Activation Protein Kinase C/metabolism T-Lymphocytes, Cytotoxic/*immunology
Pubmed
Création de la notice
28/01/2008 12:13
Dernière modification de la notice
03/03/2018 18:19
Données d'usage