Occurrence and biosynthesis of endogenous cannabinoid precursor, N-arachidonoyl phosphatidylethanolamine, in rat brain

Détails

ID Serval
serval:BIB_731DBE94820F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Occurrence and biosynthesis of endogenous cannabinoid precursor, N-arachidonoyl phosphatidylethanolamine, in rat brain
Périodique
J Neurosci
Auteur(s)
Cadas H., di Tomaso E., Piomelli D.
ISSN
0270-6474 (Print)
ISSN-L
0270-6474
Statut éditorial
Publié
Date de publication
1997
Volume
17
Numéro
4
Pages
1226-42
Langue
anglais
Notes
Cadas, H
di Tomaso, E
Piomelli, D
eng
Research Support, Non-U.S. Gov't
J Neurosci. 1997 Feb 15;17(4):1226-42.
Résumé
It has been suggested that anandamide (N-arachidonoylethanolamine), an endogenous cannabinoid substance, may be produced through Ca2+-stimulated hydrolysis of the phosphatidylethanolamine (PE) derivative N-arachidonoyl PE. The presence of N-arachidonoyl PE in adult brain tissue and the enzyme pathways that underlie its biosynthesis are, however, still undetermined. We report here that rat brain tissue contains both anandamide (11 +/- 7 pmol/gm wet tissue) and N-arachidonoyl PE (22 +/- 16 pmol/gm), as assessed by gas chromatography/mass spectrometry. We describe a N-acyltransferase activity in brain that catalyzes the biosynthesis of N-arachidonoyl PE by transferring an arachidonate group from the sn-1 carbon of phospholipids to the amino group of PE. We also show that sn-1 arachidonoyl phospholipids are present in brain, where they constitute approximately 0.5% of total phospholipids. N-acyltransferase activity is Ca2+ dependent and is enriched in brain and testis. Within the brain, N-acyltransferase activity is highest in brainstem; intermediate in cortex, striatum, hippocampus, medulla, and cerebellum; and lowest in thalamus, hypothalamus, and olfactory bulb. Pharmacological inhibition of N-acyltransferase activity in primary cultures of cortical neurons prevents Ca2+-stimulated N-arachidonoyl PE biosynthesis. Our results demonstrate, therefore, that rat brain tissue contains the complement of enzymatic activity and lipid substrates necessary for the biosynthesis of the anandamide precursor N-arachidonoyl PE. They also suggest that biosynthesis of N-arachidonoyl PE and formation of anandamide are tightly coupled processes, which may concomitantly be stimulated by elevations in intracellular Ca2+ occurring during neural activity.
Mots-clé
Acyltransferases/metabolism, Animals, Arachidonic Acids/metabolism, Brain/*metabolism, Cannabinoids/*metabolism, Endocannabinoids, Enzymes/metabolism, Lipid Metabolism, Male, Phosphatidylethanolamines/*metabolism, Phospholipids/metabolism, Polyunsaturated Alkamides, Prodrugs/*metabolism, Rats, Rats, Wistar, Tissue Distribution
Pubmed
Création de la notice
13/11/2017 10:57
Dernière modification de la notice
11/08/2018 6:26
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