A recombinant Salmonella typhimurium vaccine induces local immunity by four different routes of immunization

Détails

ID Serval
serval:BIB_730967336BBC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A recombinant Salmonella typhimurium vaccine induces local immunity by four different routes of immunization
Périodique
Infection and Immunity
Auteur(s)
Hopkins  S., Kraehenbuhl  J. P., Schodel  F., Potts  A., Peterson  D., de Grandi  P., Nardelli-Haefliger  D.
ISSN
0019-9567 (Print)
Statut éditorial
Publié
Date de publication
09/1995
Volume
63
Numéro
9
Pages
3279-86
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Sep
Résumé
Immunization of mice with an attenuated Salmonella typhimurium strain (Phopc) carrying a plasmid encoding a hybrid form of the hepatitis B virus core antigen (HBc) induced specific antibody responses against the bacterial lipopolysaccharide (LPS) and HBc. Different mucosal routes of immunization, i.e., oral, nasal, rectal, and vaginal, were compared for their ability to induce a systemic as well as a mucosal response at sites proximal or distant to the site of immunization. Anti-LPS and anti-HBc immunoglobulin A (IgA) antibodies were measured in saliva, in feces, and in genital, bronchial, and intestinal secretions. Specific antibodies in serum and secretions were observed after immunization via all routes; however, the response to LPS was independent of that against HBc. In serum, saliva, and genital and bronchial secretions, high amounts of anti-HBc IgA were obtained by the nasal route of immunization. Vaginal immunization resulted in two different responses in mice: high and low. We observed a correlation between the level of specific immune response and the estrous status of these mice at the time of immunization. Rectal immunization induced high amounts of IgA against HBc and LPS in colonorectal secretions and feces but not at distant sites. These data suggest that S. typhimurium is able to invade different mucosal tissues and induce long-lasting local IgA responses against itself and a carried antigen after a single immunization.
Mots-clé
Animals Antibodies, Bacterial/biosynthesis Bacterial Vaccines/administration & dosage/*immunology Female Hepatitis B Core Antigens/immunology Immunization Immunoglobulin A, Secretory/biosynthesis Immunoglobulin G/biosynthesis Intestinal Mucosa/immunology Lipopolysaccharides/immunology Mice Mice, Inbred BALB C Rabbits Salmonella typhimurium/*immunology Vaccines, Synthetic/*immunology
Pubmed
Web of science
Création de la notice
25/01/2008 16:05
Dernière modification de la notice
03/03/2018 18:18
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