Expression patterns of loricrin in various species and tissues

Détails

ID Serval
serval:BIB_72F380A2B5ED
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Expression patterns of loricrin in various species and tissues
Périodique
Differentiation
Auteur(s)
Hohl  D., Ruf Olano  B., de Viragh  P. A., Huber  M., Detrisac  C. J., Schnyder  U. W., Roop  D. R.
ISSN
0301-4681 (Print)
Statut éditorial
Publié
Date de publication
08/1993
Volume
54
Numéro
1
Pages
25-34
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Aug
Résumé
In this study we analyzed the expression patterns of loricrin in various species and tissues using immunohistochemistry, immunoblotting and Northern blots. Loricrin is a glycine-, serine- and cysteine-rich protein expressed very late in epidermal differentiation in the granular layers of normal mouse and human epidermis. Later on in differentiation, loricrin becomes crosslinked as a major component into the cornified cell envelope by the formation of N epsilon-(gamma-glutamyl)lysine isopeptide bonds. This process either occurs directly or by the intermediate accumulation in L-keratohyaline granules of mouse epidermis and human acrosyringia. Loricrin was identified in all mammalian species analyzed by virtue of its highly conserved carboxy-terminal sequences revealing an electric mobility of approximately 60 kDa in rodents, rabbit and cow and of approximately 35 kDa in lamb and human on sodium dodecyl sulfate polyacrylamide gel electrophoresis. Loricrin is expressed in the granular layer of all mammalian orthokeratinizing epithelia tested including oral, esophageal and fore-stomach mucosa of rodents, tracheal squamous metaplasia of vitamin A deficient hamster and estrogen induced squamous vaginal epithelium of ovary ectomized rats. Loricrin is also expressed in a few parakeratinizing epithelia such as BBN [N-butyl-N-(4-hydroxybutyl)nitrosamine]-induced murine bladder carcinoma and a restricted subset of oral and single vaginal epithelial cells in higher mammals. Our results provide further evidence that the program of squamous differentiation in internal epithelia of the upper alimentary tract in rodents and higher mammals differ remarkably. In addition, we also have noted the distinct distribution patterns of human loricrin and involucrin, another major precursor protein of the cornified cell envelope.
Mots-clé
Animals Butylhydroxybutylnitrosamine Cell Differentiation/physiology Cricetinae Female Humans Male Membrane Proteins/*biosynthesis Mesocricetus Metaplasia/etiology Mice Mice, Inbred Strains Organ Specificity/physiology Rabbits Rats Rats, Inbred F344 Species Specificity Trachea/pathology Urinary Bladder Neoplasms/chemically induced Vagina/pathology Vitamin A Deficiency/complications/pathology
Pubmed
Web of science
Création de la notice
25/01/2008 17:36
Dernière modification de la notice
03/03/2018 18:18
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