Human large granular lymphocytes contain an esterase activity usually considered as specific for the myeloid series.

Détails

ID Serval
serval:BIB_72DB625A1DDE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Human large granular lymphocytes contain an esterase activity usually considered as specific for the myeloid series.
Périodique
European Journal of Immunology
Auteur(s)
Heumann D., Colombatti M., Mach J.P.
ISSN
0014-2980 (Print)
ISSN-L
0014-2980
Statut éditorial
Publié
Date de publication
1983
Volume
13
Numéro
3
Pages
254-258
Langue
anglais
Résumé
A cytochemical marker such as alpha-naphthyl acetate esterase (ANAE) has been found useful for the morphological identification of the subset of T lymphocytes having receptors for Fcμ (TM cells). ANAE reaction on TM cells gives a typical pattern of one to four positive spots, whereas this pattern is not found on T cells with receptors for Fcγ (TG cells). ANAE is abundant in monocytes but not detectable in granulocytes. Herein another type of esterase activity, naphthol-AS-D chloroacetate esterase (NCAE), is described; it is known to be abundant in granulocytes and was found to give a specific pattern of reactivity with the subpopulation of large granular lymphocytes (LGL). This pattern of fine granular staining was observed not only on LGL present in the TG cell subpopulation but also in LGL present in the non-T, non-B cells.
Fractions of peripheral blood mononuclear cells which were ènriched up to 80% in LGL by Percoll discontinuous density gradient gave a similar percentage of specific NCAE pattern. In addition, among the different fractions from Percoll gradient, there was a good correlation (r = 0.94) between the number of NCAE-positive cells and the natural killer activity against the natural killer susceptible K562 target cells. It will be important to determine whether or not this enzymatic activity plays a role in the cytotoxic activities of LGL.
Mots-clé
Adult, Carboxylic Ester Hydrolases/blood, Cell Line, Cytotoxicity, Immunologic, Humans, Killer Cells, Natural/immunology, Leukemia, Lymphoid/immunology, Lymphocytes/cytology, Lymphocytes/enzymology, Naphthol AS D Esterase/blood, Staining and Labeling, T-Lymphocytes/enzymology
Pubmed
Web of science
Création de la notice
25/04/2013 14:49
Dernière modification de la notice
03/03/2018 18:18
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