Technologies of directed protein evolution in vivo.

Détails

Ressource 1Télécharger: BIB_72C4906655F8.P001.pdf (392.40 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_72C4906655F8
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Titre
Technologies of directed protein evolution in vivo.
Périodique
Cellular and Molecular Life Sciences
Auteur(s)
Blagodatski A., Katanaev V.L.
ISSN
1420-9071 (Electronic)
ISSN-L
1420-682X
Statut éditorial
Publié
Date de publication
2011
Volume
68
Numéro
7
Pages
1207-1214
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Review
Résumé
Directed evolution of proteins for improved or modified functionality is an important branch of modern biotechnology. It has traditionally been performed using various in vitro methods, but more recently, methods of in vivo artificial evolution come into play. In this review, we discuss and compare prokaryotic and eukaryotic-based systems of directed protein evolution in vivo, highlighting their benefits and current limitations and focusing on the biotechnological potential of vertebrate immune cells for the generation of protein diversity by means of the immunoglobulin diversification machinery.
Mots-clé
Animals, Directed Molecular Evolution/methods, Eukaryota/genetics, Eukaryota/metabolism, Humans, Prokaryotic Cells/physiology, Protein Engineering/methods, Proteins/genetics
Pubmed
Création de la notice
22/12/2011 17:10
Dernière modification de la notice
03/03/2018 18:17
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