Costimulation provided by DNA immunization enhances antitumor immunity
Details
Serval ID
serval:BIB_7281EC9A3277
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Costimulation provided by DNA immunization enhances antitumor immunity
Journal
Journal of Immunology
ISSN
0022-1767 (Print)
Publication state
Published
Issued date
11/1997
Volume
159
Number
10
Pages
4999-5004
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Nov 15
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Nov 15
Abstract
The interaction of the TCR with MHC class I-bound Ag is insufficient for the priming of CTL unless secondary costimulatory signals are provided. To ascertain the minimum elements required to activate an Ag-specific CTL response in vivo, we injected mice intradermally or i.m. with plasmid DNA encoding a MHC class I-restricted peptide Ag (minigene) and different membrane-bound costimulatory ligands. The minigene-encoded epitope only primed a specific CTL response if injected in the vicinity of an ectopically expressed costimulatory ligand. Vector encoding B7-1 was repeatedly more potent at stimulating a cytolytic response than vector encoding B7-2. In contrast the B7-2-encoding plasmid preferentially enhanced Ag-specific Ab responses when injected with either protein or a cDNA expression vector. Gene vaccination with plasmids encoding OVA and B7-1, but not B7-2, prolonged survival in mice challenged with an OVA-transfected tumor. These results show that functional B7-1 transfection can be achieved in vivo and induces the selective induction of CTL. The data suggest that B7-1 plasmids should be coadministered with naked DNA vaccines that aim to induce tumor-specific cellular immunity.
Keywords
Animals
Antigens, CD/biosynthesis/physiology
Antigens, CD86
DNA/*immunology
Epitopes, T-Lymphocyte/genetics/immunology
Female
Histocompatibility Antigens Class I/genetics
Immunoglobulin G/biosynthesis
Ligands
*Lymphocyte Activation/genetics
Membrane Glycoproteins/biosynthesis/physiology
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Neoplasm Transplantation
Ovalbumin/immunology
Plasmids/immunology
Sarcoma, Experimental
T-Lymphocytes, Cytotoxic/immunology
Thymoma
Tumor Cells, Cultured
Tumor Escape/*genetics/immunology
Pubmed
Web of science
Create date
25/01/2008 9:31
Last modification date
20/08/2019 15:30