Humoral and cellular immune response to a Microsporum canis recombinant keratinolytic metalloprotease (r-MEP3) in experimentally infected guinea pigs

Détails

ID Serval
serval:BIB_7271A7D5BE6D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Humoral and cellular immune response to a Microsporum canis recombinant keratinolytic metalloprotease (r-MEP3) in experimentally infected guinea pigs
Périodique
Medical Mycology
Auteur(s)
Brouta  F., Descamps  F., Vermout  S., Monod  M., Losson  B., Mignon  B.
ISSN
1369-3786 (Print)
Statut éditorial
Publié
Date de publication
12/2003
Volume
41
Numéro
6
Pages
495-501
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec
Résumé
In order to better understand the host-fungus relationship in Microsporum canis dermatophytosis and to identify major fungal antigens, the immune response to a crude exoantigen preparation and to a purified recombinant keratinolytic metalloprotease (r-MEP3) was evaluated in guinea pigs experimentally infected with M. canis. Humoral and cellular immune responses were assessed from day 0 to day 57 post-infection (PI), the former by enzyme-linked immunosorbent assay (ELISA) and the latter via a lymphocyte proliferation assay. Infected guinea pigs developed humoral and cellular responses to both M. canis exoantigen and r-MEP3, while no specific immune response to these antigens was observed in control animals. This is the first report on the development of both humoral and cell-mediated immune responses to a purified keratinase in M. canis dermatophytosis.
Mots-clé
Animal Experimentation Animals Antibodies, Fungal/*blood Antibody Formation/*immunology Antigens, Fungal/immunology Dermatomycoses/blood/*immunology Enzyme-Linked Immunosorbent Assay Guinea Pigs Immunity, Cellular/*immunology Metalloproteases/analysis/*immunology Microsporum/*enzymology/immunology Peptide Hydrolases/metabolism Recombinant Proteins/immunology
Pubmed
Web of science
Création de la notice
25/01/2008 17:47
Dernière modification de la notice
03/03/2018 18:17
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