Expression of T cell receptor genes in the thymus: localization of transcripts in situ and comparison of mature and immature subsets.

Détails

ID Serval
serval:BIB_726E8EEBC7BA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Expression of T cell receptor genes in the thymus: localization of transcripts in situ and comparison of mature and immature subsets.
Périodique
European journal of immunology
Auteur(s)
Held W., Mueller C., MacDonald H.R.
ISSN
0014-2980
Statut éditorial
Publié
Date de publication
1990
Peer-reviewed
Oui
Volume
20
Numéro
9
Pages
2133-2136
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
We have analyzed the expression of T cell receptor (TcR) genes in the thymus using in situ RNA hybridizations with probes to the constant regions of the TcR alpha, beta, gamma and delta chains. Localization of transcripts revealed low TcR alpha mRNA levels in the thymus cortex and very low levels in the subcapsular region. In contrast, TcR beta message was very abundant in the cortex. TcR gamma or delta mRNA+ thymocytes showed a scattered, predominantly cortical localization. In contrast to gamma, TcR delta transcripts were abundant in the subcapsular region. Control experiments with sorted TcR alpha/beta or gamma/delta cells revealed a detection efficiency of 75%-85% for the respective TcR mRNA and data on TcR gene expression in mature, CD3+ thymocytes were consistent with previous reports. The analysis of immature, CD3- thymocyte subsets, however, revealed a virtual absence of TcR alpha transcripts and an unexpectedly high proportion of cells (14%-29%) expressing the gene for the TcR delta chain. The data are discussed in view of current models of lineage relationships in the thymus.
Mots-clé
Animals, Antigens, CD4/analysis, Antigens, CD8, Antigens, Differentiation, T-Lymphocyte/analysis, Gene Expression, Mice, Mice, Inbred C57BL, Nucleic Acid Hybridization, RNA, Messenger/analysis, Receptors, Antigen, T-Cell/genetics, T-Lymphocyte Subsets/immunology, Thymus Gland/immunology
Pubmed
Web of science
Création de la notice
17/01/2008 16:24
Dernière modification de la notice
03/03/2018 18:17
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