Mycobacterium tuberculosis-specific CD8+ T cells are functionally and phenotypically different between latent infection and active disease.

Détails

ID Serval
serval:BIB_7269592AD615
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Mycobacterium tuberculosis-specific CD8+ T cells are functionally and phenotypically different between latent infection and active disease.
Périodique
European Journal of Immunology
Auteur(s)
Rozot V., Vigano S., Mazza-Stalder J., Idrizi E., Day C.L., Perreau M., Lazor-Blanchet C., Petruccioli E., Hanekom W., Goletti D., Bart P.A., Nicod L., Pantaleo G., Harari A.
ISSN
1521-4141 (Electronic)
ISSN-L
0014-2980
Statut éditorial
Publié
Date de publication
2013
Volume
43
Numéro
6
Pages
1568-1577
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Résumé
Protective immunity to Mycobacterium tuberculosis (Mtb) remains poorly understood and the role of Mtb-specific CD8(+) T cells is controversial. Here we performed a broad phenotypic and functional characterization of Mtb-specific CD8(+) T cells in 326 subjects with latent Mtb infection (LTBI) or active TB disease (TB). Mtb-specific CD8(+) T cells were detected in most (60%) TB patients and few (15%) LTBI subjects but were of similar magnitude. Mtb-specific CD8(+) T cells in LTBI subjects were mostly T EMRA cells (CD45RA(+) CCR7(-)), coexpressing 2B4 and CD160, and in TB patients were mostly TEM cells (CD45RA(-) CCR7(-)), expressing 2B4 but lacking PD-1 and CD160. The cytokine profile was not significantly different in both groups. Furthermore, Mtb-specific CD8(+) T cells expressed low levels of perforin and granulysin but contained granzymes A and B. However, in vitro-expanded Mtb-specific CD8(+) T cells expressed perforin and granulysin. Finally, Mtb-specific CD8(+) T-cell responses were less frequently detected in extrapulmonary TB compared with pulmonary TB patients. Mtb-specific CD8(+) T-cell proliferation was also greater in patients with extrapulmonary compared with pulmonary TB. Thus, the activity of Mtb infection and clinical presentation are associated with distinct profiles of Mtb-specific CD8(+) T-cell responses. These results provide new insights in the interaction between Mtb and the host immune response.
Mots-clé
Acute Disease, Antigens, CD/metabolism, CD8-Positive T-Lymphocytes/immunology, Cell Proliferation, Cells, Cultured, Cytokines/metabolism, GPI-Linked Proteins/metabolism, Humans, Immunophenotyping, Latent Tuberculosis/immunology, Mycobacterium tuberculosis/immunology, Programmed Cell Death 1 Receptor/metabolism, Receptors, Immunologic/metabolism, T-Lymphocyte Subsets/immunology, Tuberculosis, Pulmonary/immunology
Pubmed
Web of science
Création de la notice
24/06/2013 14:48
Dernière modification de la notice
03/03/2018 18:17
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