Two functional forms of the Xenopus laevis estrogen receptor translated from a single mRNA species.

Details

Serval ID
serval:BIB_725B382E9B00
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Two functional forms of the Xenopus laevis estrogen receptor translated from a single mRNA species.
Journal
Journal of Biological Chemistry
Author(s)
Claret F.X., Chapel S., Garcés J., Tsai-Pflugfelder M., Bertholet C., Shapiro D.J., Wittek R., Wahli W.
ISSN
0021-9258[print], 0021-9258[linking]
Publication state
Published
Issued date
05/1994
Volume
269
Number
19
Pages
14047-14055
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Steroid receptors are nuclear proteins that regulate gene transcription in a ligand-dependent manner. Over-expression of the Xenopus estrogen receptor in a vaccinia virus-derived expression system revealed that the receptor localized exclusively in the nucleus of the infected cells, irrespective of the presence or absence of the ligand. Furthermore, two forms of the receptor were produced, a full-length and a N-terminal truncated version, which are translated from a single mRNA species by the use of two AUG within the same reading frame. These 66- and 61-kDa receptors were also observed after in vitro translation of the mRNA as well as in primary Xenopus hepatocytes. Both forms are potent estrogen-dependent transcriptional activators in transient transfection experiments, as well as in in vitro transcription assays.
Keywords
Animals, Base Sequence, DNA/metabolism, DNA-Binding Proteins/genetics, DNA-Binding Proteins/metabolism, Hela Cells, Humans, Liver/cytology, Liver/metabolism, Molecular Sequence Data, Protein Biosynthesis, RNA, Messenger/genetics, RNA, Messenger/metabolism, Receptors, Estrogen/genetics, Receptors, Estrogen/metabolism, Recombinant Proteins/genetics, Recombinant Proteins/metabolism, Recombination, Genetic, Transcriptional Activation, Vaccinia virus/genetics, Xenopus laevis
Pubmed
Web of science
Create date
24/01/2008 10:43
Last modification date
20/08/2019 14:30
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