Two functional forms of the Xenopus laevis estrogen receptor translated from a single mRNA species.

Détails

ID Serval
serval:BIB_725B382E9B00
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Two functional forms of the Xenopus laevis estrogen receptor translated from a single mRNA species.
Périodique
Journal of Biological Chemistry
Auteur(s)
Claret F.X., Chapel S., Garcés J., Tsai-Pflugfelder M., Bertholet C., Shapiro D.J., Wittek R., Wahli W.
ISSN
0021-9258[print], 0021-9258[linking]
Statut éditorial
Publié
Date de publication
05/1994
Volume
269
Numéro
19
Pages
14047-14055
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Steroid receptors are nuclear proteins that regulate gene transcription in a ligand-dependent manner. Over-expression of the Xenopus estrogen receptor in a vaccinia virus-derived expression system revealed that the receptor localized exclusively in the nucleus of the infected cells, irrespective of the presence or absence of the ligand. Furthermore, two forms of the receptor were produced, a full-length and a N-terminal truncated version, which are translated from a single mRNA species by the use of two AUG within the same reading frame. These 66- and 61-kDa receptors were also observed after in vitro translation of the mRNA as well as in primary Xenopus hepatocytes. Both forms are potent estrogen-dependent transcriptional activators in transient transfection experiments, as well as in in vitro transcription assays.
Mots-clé
Animals, Base Sequence, DNA/metabolism, DNA-Binding Proteins/genetics, DNA-Binding Proteins/metabolism, Hela Cells, Humans, Liver/cytology, Liver/metabolism, Molecular Sequence Data, Protein Biosynthesis, RNA, Messenger/genetics, RNA, Messenger/metabolism, Receptors, Estrogen/genetics, Receptors, Estrogen/metabolism, Recombinant Proteins/genetics, Recombinant Proteins/metabolism, Recombination, Genetic, Transcriptional Activation, Vaccinia virus/genetics, Xenopus laevis
Pubmed
Web of science
Création de la notice
24/01/2008 11:43
Dernière modification de la notice
03/03/2018 18:17
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