The human locus coeruleus complex: an immunohistochemical and three dimensional reconstruction study

Details

Serval ID
serval:BIB_721253531130
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The human locus coeruleus complex: an immunohistochemical and three dimensional reconstruction study
Journal
Experimental Brain Research
Author(s)
Baker  K. G., Tork  I., Hornung  J. P., Halasz  P.
ISSN
0014-4819 (Print)
Publication state
Published
Issued date
1989
Volume
77
Number
2
Pages
257-70
Notes
Journal Article
Research Support, Non-U.S. Gov't
Abstract
The CA (catecholamine/catecholaminergic) cell populations of the locus coeruleus (LC) and subcoeruleus (SubC) were studied using serial sections of the human brainstem immunostained with an antibody against tyrosine hydroxylase. The tyrosine hydroxylase-immunoreactive (TH-IR) neurons were plotted in a computer reconstruction system and their number and soma size determined. Serial section computer analysis was then used to create a three dimensional reconstruction of the LC complex. The number of cells containing neuromelanin pigment was also determined and compared with the number of TH-IR cells. In our sample there were 53,900 TH-IR cells in the LC and a further 6260 cells in the SubC. These numbers were very similar to our estimates of the number of cells containing neuromelanin pigment and we concluded that virtually all of these cells were also tyrosine hydroxylase positive. The average soma size of the TH-IR cells of the LC was 37 microns and in the SubC 34 microns. In addition to these quantitative observations the morphology of the TH-IR and the Nissl stained cells is described in some detail. We also compared the groups of immunoreactive cells in the human pons with the noradrenergic groups A5-A7 described in the rat. Although in the human these groups are contiguous, A5 is not part of the LC complex. However we did find that the A7 group is equivalent to the rostroventral part of SubC while the remainder of SubC is formed by ventral A6.
Keywords
Humans *Image Processing, Computer-Assisted Immunohistochemistry Locus Coeruleus/analysis/*cytology Melanins/analysis Neurons/analysis Tyrosine 3-Monooxygenase/analysis
Pubmed
Web of science
Create date
24/01/2008 14:22
Last modification date
20/08/2019 14:30
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