Marginal role of von Willebrand factor-binding protein and coagulase in the initiation of endocarditis in rats with catheter-induced aortic vegetations.

Détails

ID Serval
serval:BIB_714C89A05524
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Marginal role of von Willebrand factor-binding protein and coagulase in the initiation of endocarditis in rats with catheter-induced aortic vegetations.
Périodique
Virulence
Auteur(s)
Mancini S., Oechslin F., Menzi C., Que Y.A., Claes J., Heying R., Veloso T.R., Vanassche T., Missiakas D., Schneewind O., Moreillon P., Entenza J.M.
ISSN
2150-5608 (Electronic)
ISSN-L
2150-5594
Statut éditorial
Publié
Date de publication
2018
Peer-reviewed
Oui
Volume
9
Numéro
1
Pages
1615-1624
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Staphylococcus aureus is the leading cause of infective endocarditis (IE). While the role of S. aureus cell-wall associated protein clumping factor A (ClfA) in promoting IE has been already demonstrated, that of the secreted plasma-clotting factors staphylocoagulase (Coa) and von Willebrand factor-binding protein (vWbp) has not yet been elucidated. We investigated the role of Coa and vWbp in IE initiation in rats with catheter-induced aortic vegetations, using Lactococcus lactis expressing coa, vWbp, clfA or vWbp/clfA, and S. aureus Newman Δcoa, ΔvWbp, ΔclfA or Δcoa/ΔvWbp/ΔclfA mutants. vWbp-expression increased L. lactis valve infection compared to parent and coa-expressing strains (incidence: 62%, versus 0% and 13%, respectively; P < 0.01). Likewise, expression of clfA increased L. lactis infectivity (incidence: 80%), which was not further affected by co-expression of vWbp. In symmetry, deletion of the coa or vWbp genes in S. aureus did not decrease infectivity (incidence: 68 and 64%, respectively) whereas deletion of clfA did decrease valve infection (incidence: 45%; P = 0.03 versus parent), which was not further affected by the triple deletion Δcoa/ΔvWbp/ΔclfA (incidence: 36%; P > 0.05 versus ΔclfA mutant). Coa does not support the initial colonization of IE (in L. lactis) without other key virulence factors and vWbp contributes to initiation of IE (in L. lactis) but is marginal in the present of ClfA.
Mots-clé
Animals, Aortic Valve/microbiology, Aortic Valve/physiopathology, Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Catheter-Related Infections/microbiology, Coagulase/genetics, Coagulase/metabolism, Endocarditis, Bacterial/pathology, Female, Gene Deletion, Lactococcus lactis/genetics, Lactococcus lactis/metabolism, Rats, Rats, Wistar, Staphylococcal Infections, Staphylococcus aureus/genetics, Staphylococcus aureus/pathogenicity, Virulence Factors/genetics, von Willebrand Factor/metabolism, Clumping factor A, Staphylococcus aureus, endocarditis, staphylocoagulase, von Willebrand factor-binding protein
Pubmed
Web of science
Open Access
Oui
Création de la notice
16/10/2018 13:11
Dernière modification de la notice
20/08/2019 15:29
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