Bartonella apis sp. nov., a honey bee gut symbiont of the class Alphaproteobacteria.

Détails

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Etat: Serval
Version: Author's accepted manuscript
ID Serval
serval:BIB_714B2174AB90
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Bartonella apis sp. nov., a honey bee gut symbiont of the class Alphaproteobacteria.
Périodique
International Journal of Systematic and Evolutionary Microbiology
Auteur(s)
Kešnerová L., Moritz R., Engel P.
ISSN
1466-5034 (Electronic)
ISSN-L
1466-5026
Statut éditorial
Publié
Date de publication
2016
Peer-reviewed
Oui
Volume
66
Numéro
1
Pages
414-421
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Here, we report the culture and characterization of an alphaproteobacterium of the order Rhizobiales, isolated from the gut of the honey bee Apis mellifera. Strain PEB0122T shares >95 % 16S rRNA gene sequence similarity with species of the genus Bartonella, a group of mammalian pathogens transmitted by bloodsucking arthropods. Phylogenetic analyses showed that PEB0122T and related strains from the honey bee gut form a sister clade of the genus Bartonella. Optimal growth of strain PEB0122T was obtained on solid media supplemented with defibrinated sheep blood under microaerophilic conditions at 35-37 °C, which is consistent with the cultural characteristics of other species of the genus Bartonella. Reduced growth of strain PEB0122T also occurred under aerobic conditions. The rod-shaped cells of strain PEB0122T had a mean length of 1.2-1.8 μm and revealed hairy surface structures. Strain PEB0122T was positive for catalase, cytochrome c oxidase, urease and nitrate reductase. The fatty acid composition was comparable to those of other species of the genus Bartonella, with palmitic acid (C16 : 0) and isomers of 18- and 19-carbon chains being the most abundant. The genomic DNA G+C content of PEB0122T was determined to be about 45.5 mol%. The high 16S rRNA gene sequence similarity with species of Bartonella and its close phylogenetic position suggest that strain PEB0122T represents a novel species within the genus Bartonella, for which we propose the name Bartonella apis sp. nov. The type strain is PEB0122T ( = NCIMB 14961T = DSM 29779T).
Mots-clé
Animals, Bacterial Typing Techniques, Bartonella/classification, Bartonella/genetics, Base Composition, Bees/microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Gastrointestinal Tract/microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Symbiosis
Pubmed
Web of science
Open Access
Oui
Création de la notice
15/01/2016 12:52
Dernière modification de la notice
08/05/2019 20:18
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