Article: article from journal or magazin.
IL-12 administration leads to a transient depletion of T cells, B cells, and APCs and concomitant abrogation of the HLA-A2.1-restricted CTL response in transgenic mice
Journal of Immunology
Journal Article --- Old month value: Jul 1
The injection of a mixture of bona fide T cell epitopes can lead to the occurrence of immunodominance, meaning that the immune response is focused on the recognition of a single epitope or a small portion of the epitopes injected. We have previously demonstrated that the administration of rIL-12 can counteract immunodominance in BALB/c mice. In this study, we show that the administration of rIL-12 to HLA-A2.1 transgenic mice (A2k(b) mice) abrogates specifically the immune response against HLA-A2.1-restricted HIV epitopes in the spleen. This lack of immune response is most probably due to a transient depletion of B cells, T cells, macrophages, and dendritic cells in this organ. Therefore, our study explains the mechanism of immunosuppression by rIL-12 in vivo.
AIDS Vaccines/administration & dosage/immunology Animals Antigen-Presenting Cells/*immunology B-Lymphocytes/*immunology Cells, Cultured Cytotoxicity, Immunologic/*genetics Down-Regulation/genetics/immunology Epitopes, T-Lymphocyte/genetics/immunology Female H-2 Antigens/genetics HLA-A2 Antigen/biosynthesis/*genetics/immunology Immunodominant Epitopes/genetics/immunology Injections, Intraperitoneal Interleukin-12/*administration & dosage Lymphocyte Depletion/*methods Male Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Transgenic Oligopeptides/administration & dosage/immunology Recombinant Proteins/administration & dosage Spleen/cytology/immunology/metabolism T-Lymphocytes/*immunology
Web of science
Last modification date