An elaborate classification of SNARE proteins sheds light on the conservation of the eukaryotic endomembrane system.

Details

Serval ID
serval:BIB_6FEC04F1F801
Type
Article: article from journal or magazin.
Collection
Publications
Title
An elaborate classification of SNARE proteins sheds light on the conservation of the eukaryotic endomembrane system.
Journal
Molecular Biology of the Cell
Author(s)
Kloepper T.H., Kienle C.N., Fasshauer D.
ISSN
1059-1524 (Print)
ISSN-L
1059-1524
Publication state
Published
Issued date
2007
Peer-reviewed
Oui
Volume
18
Number
9
Pages
3463-3471
Language
english
Abstract
Proteins of the SNARE (soluble N-ethylmalemide-sensitive factor attachment protein receptor) family are essential for the fusion of transport vesicles with an acceptor membrane. Despite considerable sequence divergence, their mechanism of action is conserved: heterologous sets assemble into membrane-bridging SNARE complexes, in effect driving membrane fusion. Within the cell, distinct functional SNARE units are involved in different trafficking steps. These functional units are conserved across species and probably reflect the conservation of the particular transport step. Here, we have systematically analyzed SNARE sequences from 145 different species and have established a highly accurate classification for all SNARE proteins. Principally, all SNAREs split into four basic types, reflecting their position in the four-helix bundle complex. Among these four basic types, we established 20 SNARE subclasses that probably represent the original repertoire of a eukaryotic cenancestor. This repertoire has been modulated independently in different lines of organisms. Our data are in line with the notion that the ur-eukaryotic cell was already equipped with the various compartments found in contemporary cells. Possibly, the development of these compartments is closely intertwined with episodes of duplication and divergence of a prototypic SNARE unit.
Keywords
Animals, Caenorhabditis elegans, Cell Membrane/metabolism, Conserved Sequence, Drosophila melanogaster, Eukaryotic Cells/cytology, Evolution, Molecular, Humans, Phylogeny, Protein Structure, Tertiary, SNARE Proteins/chemistry, SNARE Proteins/classification
Pubmed
Web of science
Create date
15/09/2011 8:18
Last modification date
20/08/2019 14:28
Usage data