Regulation of hepatokine gene expression in response to fasting and feeding: Influence of PPAR-α and insulin-dependent signalling in hepatocytes.
Details
Serval ID
serval:BIB_6FBA3465D601
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Regulation of hepatokine gene expression in response to fasting and feeding: Influence of PPAR-α and insulin-dependent signalling in hepatocytes.
Journal
Diabetes & metabolism
ISSN
1878-1780 (Electronic)
ISSN-L
1262-3636
Publication state
Published
Issued date
04/2020
Peer-reviewed
Oui
Volume
46
Number
2
Pages
129-136
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
In hepatocytes, the peroxisome proliferator-activated receptor (PPAR)-α and insulin receptor (IR) are critical for transcriptional responses to fasting and feeding, respectively. The present report analyzes the effects of nutritional status (fasting vs feeding) on the expression of a large panel of hepatokines in hepatocyte-specific PPAR-α (Pparα <sup>hep-/-</sup> ) and IR (IR <sup>hep-/-</sup> ) null mice.
Pparα <sup>hep-/-</sup> and IR <sup>hep-/-</sup> mice, and their wild-type littermates, were subjected to fasting or feeding metabolic challenges, then analyzed for hepatokine gene expression. Experiments were conducted in mice of both genders.
Our data confirmed that PPAR-α is essential for regulating fasting-induced Fgf21 and Angptl4 expression. In mice lacking PPAR-α, fasting led to increased Igfbp1 and Gdf15 gene expression. In the absence of hepatic IR, feeding induced overexpression of Igfbp1, follistatin (Fst) and adropin (Enho), and reduced activin E (Inhbe) expression. Gender had only a modest influence on hepatokine gene expression in the liver.
The present results highlight the potential roles of hepatokines as a class of hormones that substantially influence nutritional regulation in both female and male mice.
Pparα <sup>hep-/-</sup> and IR <sup>hep-/-</sup> mice, and their wild-type littermates, were subjected to fasting or feeding metabolic challenges, then analyzed for hepatokine gene expression. Experiments were conducted in mice of both genders.
Our data confirmed that PPAR-α is essential for regulating fasting-induced Fgf21 and Angptl4 expression. In mice lacking PPAR-α, fasting led to increased Igfbp1 and Gdf15 gene expression. In the absence of hepatic IR, feeding induced overexpression of Igfbp1, follistatin (Fst) and adropin (Enho), and reduced activin E (Inhbe) expression. Gender had only a modest influence on hepatokine gene expression in the liver.
The present results highlight the potential roles of hepatokines as a class of hormones that substantially influence nutritional regulation in both female and male mice.
Keywords
Angiopoietin-like 4 Protein/genetics, Angiopoietin-like 4 Protein/metabolism, Animals, Eating/physiology, Fasting/metabolism, Fibroblast Growth Factors/genetics, Fibroblast Growth Factors/metabolism, Gene Expression, Hepatocytes/metabolism, Insulin/metabolism, Mice, Mice, Knockout, PPAR alpha/genetics, PPAR alpha/metabolism, Receptor, Insulin/genetics, Receptor, Insulin/metabolism, Signal Transduction/physiology, Hepatokines, Insulin receptor, Nutritional regulation, PPAR-α
Pubmed
Web of science
Create date
13/07/2021 12:30
Last modification date
16/07/2021 5:37