Regulation of hepatokine gene expression in response to fasting and feeding: Influence of PPAR-α and insulin-dependent signalling in hepatocytes.

Details

Serval ID
serval:BIB_6FBA3465D601
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Regulation of hepatokine gene expression in response to fasting and feeding: Influence of PPAR-α and insulin-dependent signalling in hepatocytes.
Journal
Diabetes & metabolism
Author(s)
Smati S., Régnier M., Fougeray T., Polizzi A., Fougerat A., Lasserre F., Lukowicz C., Tramunt B., Guillaume M., Burnol A.F., Postic C., Wahli W., Montagner A., Gourdy P., Guillou H.
ISSN
1878-1780 (Electronic)
ISSN-L
1262-3636
Publication state
Published
Issued date
04/2020
Peer-reviewed
Oui
Volume
46
Number
2
Pages
129-136
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
In hepatocytes, the peroxisome proliferator-activated receptor (PPAR)-α and insulin receptor (IR) are critical for transcriptional responses to fasting and feeding, respectively. The present report analyzes the effects of nutritional status (fasting vs feeding) on the expression of a large panel of hepatokines in hepatocyte-specific PPAR-α (Pparα <sup>hep-/-</sup> ) and IR (IR <sup>hep-/-</sup> ) null mice.
Pparα <sup>hep-/-</sup> and IR <sup>hep-/-</sup> mice, and their wild-type littermates, were subjected to fasting or feeding metabolic challenges, then analyzed for hepatokine gene expression. Experiments were conducted in mice of both genders.
Our data confirmed that PPAR-α is essential for regulating fasting-induced Fgf21 and Angptl4 expression. In mice lacking PPAR-α, fasting led to increased Igfbp1 and Gdf15 gene expression. In the absence of hepatic IR, feeding induced overexpression of Igfbp1, follistatin (Fst) and adropin (Enho), and reduced activin E (Inhbe) expression. Gender had only a modest influence on hepatokine gene expression in the liver.
The present results highlight the potential roles of hepatokines as a class of hormones that substantially influence nutritional regulation in both female and male mice.
Keywords
Angiopoietin-like 4 Protein/genetics, Angiopoietin-like 4 Protein/metabolism, Animals, Eating/physiology, Fasting/metabolism, Fibroblast Growth Factors/genetics, Fibroblast Growth Factors/metabolism, Gene Expression, Hepatocytes/metabolism, Insulin/metabolism, Mice, Mice, Knockout, PPAR alpha/genetics, PPAR alpha/metabolism, Receptor, Insulin/genetics, Receptor, Insulin/metabolism, Signal Transduction/physiology, Hepatokines, Insulin receptor, Nutritional regulation, PPAR-α
Pubmed
Web of science
Create date
13/07/2021 12:30
Last modification date
16/07/2021 5:37
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