ZAP-70 upregulation in transformed B cells after early pre-BI cell transplant into NOD/SCID mice

Details

Serval ID
serval:BIB_6FABE3D5F30D
Type
Article: article from journal or magazin.
Collection
Publications
Title
ZAP-70 upregulation in transformed B cells after early pre-BI cell transplant into NOD/SCID mice
Journal
Oncogene
Author(s)
Ruiz-Vela  A., Piqueras  R., Carvalho-Pinto  C., Gomez  L., Yaniz-Galende  E., Moreno-Ortiz  M. C., Bernad  A., Harshman  K., Martinez  A. C.
ISSN
0950-9232 (Print)
Publication state
Published
Issued date
07/2005
Volume
24
Number
32
Pages
5119-24
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul 28
Abstract
Understanding of the signal transduction pathways that lead to B cell development is of extreme interest to learn how alterations in these pathways might initiate malignant transformation. Long-term cultured early pre-BI cells can differentiate into IgM+ B cells after transplant into NOD/SCID mice, offering the possibility to explore checkpoints in B cell development. Using DNA microarray and Western blot analysis of IgM+ B cells vs parental early pre-BI cells, we demonstrated that zeta-associated protein 70 (ZAP-70) is upregulated in our B cell differentiation model. Unlike parental ZAP-70- early pre-BI cells, ZAP-70+ IgM+ B cells exhibited a transformed phenotype, as indicated by BCL-6 expression, a high Ki-67 proliferation index, resistance to IL-7 deprivation-induced apoptosis, and an increased repopulation rate in NOD/SCID mice. These data show the characterization and generation of a novel murine leukemia model with many similarities to human ZAP-70+ B cell chronic lymphocytic leukemia.
Keywords
Animals B-Lymphocytes/cytology/physiology/*transplantation Cells, Cultured Humans Leukemia, B-Cell, Chronic/genetics Mice Mice, Inbred NOD Mice, SCID Protein-Tyrosine Kinases/*genetics Signal Transduction ZAP-70 Protein-Tyrosine Kinase
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 15:33
Last modification date
20/08/2019 14:28
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