Muropeptide signature of inhibitors of protein synthesis correlates with β-lactam synergism against methicillin-resistant Staphylococcus aureus.

Détails

ID Serval
serval:BIB_6E1F621E4870
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Muropeptide signature of inhibitors of protein synthesis correlates with β-lactam synergism against methicillin-resistant Staphylococcus aureus.
Périodique
International Journal of Antimicrobial Agents
Auteur(s)
Guignard B., Majcherczyk P.A., Monachon C., Vouillamoz J., Moreillon P.
ISSN
1872-7913 (Electronic)
ISSN-L
0924-8579
Statut éditorial
Publié
Date de publication
2017
Peer-reviewed
Oui
Volume
49
Numéro
1
Pages
53-61
Langue
anglais
Résumé
Quinupristin/dalfopristin (Q/D) and β-lactams interact positively against methicillin-resistant Staphylococcus aureus (MRSA). The effect extends to other inhibitors of protein synthesis, but not to inhibitors of polynucleotide synthesis or assembly, or to Q/D plus non-β-lactam cell wall inhibitors. Moreover, electron microscopy studies have correlated this effect with a thickened cell wall. In this study, we sought to determine whether inhibitors of protein synthesis might produce a specific peptidoglycan muropeptide signature that would correlate with their positive β-lactam interaction. The muropeptides of six S. aureus isolates (three methicillin-susceptible and three MRSA) were analysed using high-performance liquid chromatography and mass spectrometry. Exposure to 0.25× the minimum inhibitory concentration of inhibitors of protein synthesis consistently produced three main alterations irrespective of methicillin resistance: (i) an increase in peak 12 (a cyclic dimer of glycine-containing disaccharide-tetrapeptide); (ii) an increase in poorly resolved late-eluting materials; and (iii) a decrease in peak 1 (a disaccharide-pentapeptide). Eventually, the rate of autolysis was also decreased, supporting the structural alteration of the peptidoglycan. Other drug classes did not produce these anomalies. An increase in peak 12 was also observed in staphylococci treated with fosfomycin, which decreases expression of the native penicillin-binding protein (PBP) 2 and 4. Parallel blockage of normal PBPs with β-lactams abolished the anomalies, indicating that they resulted from altered function of native PBPs. This underlines the potential of inhibiting both protein synthesis and transpeptidation simultaneously and suggests that such a drug combination strategy might be efficaciously exploited.

Mots-clé
Anti-Bacterial Agents/metabolism, Cell Wall/chemistry, Chromatography, High Pressure Liquid, Drug Synergism, Mass Spectrometry, Methicillin-Resistant Staphylococcus aureus/drug effects, Microbial Sensitivity Tests, Peptides/analysis, Peptidoglycan/chemistry, Protein Synthesis Inhibitors/metabolism, beta-Lactams/metabolism
Pubmed
Web of science
Création de la notice
06/12/2016 20:04
Dernière modification de la notice
03/03/2018 18:08
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