Comparison against current standards of a DNA aptamer for the label-free quantification of tobramycin in human sera employed for therapeutic drug monitoring.

Détails

ID Serval
serval:BIB_6DA135E23D6D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Comparison against current standards of a DNA aptamer for the label-free quantification of tobramycin in human sera employed for therapeutic drug monitoring.
Périodique
Journal of pharmaceutical and biomedical analysis
Auteur(s)
Tenaglia E., Ferretti A., Decosterd L.A., Werner D., Mercier T., Widmer N., Buclin T., Guiducci C.
ISSN
1873-264X (Electronic)
ISSN-L
0731-7085
Statut éditorial
Publié
Date de publication
10/09/2018
Peer-reviewed
Oui
Volume
159
Pages
341-347
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article
Publication Status: ppublish
Résumé
The use of DNA aptamers in biosensors for the quantification of pharmaceuticals in the clinics would help to overcome the limitations of antibody-based detection for small molecules. The interest for such systems is proven by the ever-increasing number of aptamer-based solutions for analytics proposed in the literature as proof-of-concept demonstrators. Despite such diversity, these platforms often lack a comparative assessment of their performances against the current standard of practice in the clinics when using real samples. We employed an aptamer against tobramycin discovered in our laboratory to quantify through surface plasmon resonance the concentration of the antibiotic in clinical samples obtained from patients treated with tobramycin and undergoing therapeutic drug monitoring. We then compared the performances of our detection strategy against the current standard of practice. Our results show how, using adequate calibration and matrix complexity reduction, DNA aptamer-based direct assays can assess clinically relevant concentrations of small molecules in patient serum and with good correlation to current standards used in the clinics.
Mots-clé
Anti-Bacterial Agents/blood, Aptamers, Nucleotide/blood, Drug Monitoring/methods, Drug Monitoring/standards, Humans, Surface Plasmon Resonance, Tobramycin/blood, DNA aptamers, Drug dosage, Drug monitoring, Label-free biosensors, SPR, Tobramycin
Pubmed
Web of science
Création de la notice
23/07/2018 23:19
Dernière modification de la notice
06/01/2019 7:26
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