Identification of Amazonian trees with DNA barcodes.

Détails

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Etat: Public
Version: Final published version
ID Serval
serval:BIB_6CEDCE30DAD5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Identification of Amazonian trees with DNA barcodes.
Périodique
PloS one
Auteur(s)
Gonzalez M.A., Baraloto C., Engel J., Mori S.A., Pétronelli P., Riéra B., Roger A., Thébaud C., Chave J.
ISSN
1932-6203[electronic]
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
4
Numéro
10
Pages
e7483
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: epublish
Résumé
BACKGROUND: Large-scale plant diversity inventories are critical to develop informed conservation strategies. However, the workload required for classic taxonomic surveys remains high and is particularly problematic for megadiverse tropical forests. METHODOLOGY/PRINCIPAL FINDINGS: Based on a comprehensive census of all trees in two hectares of a tropical forest in French Guiana, we examined whether plant DNA barcoding could contribute to increasing the quality and the pace of tropical plant biodiversity surveys. Of the eight plant DNA markers we tested (rbcLa, rpoC1, rpoB, matK, ycf5, trnL, psbA-trnH, ITS), matK and ITS had a low rate of sequencing success. More critically, none of the plastid markers achieved a rate of correct plant identification greater than 70%, either alone or combined. The performance of all barcoding markers was noticeably low in few species-rich clades, such as the Laureae, and the Sapotaceae. A field test of the approach enabled us to detect 130 molecular operational taxonomic units in a sample of 252 juvenile trees. Including molecular markers increased the identification rate of juveniles from 72% (morphology alone) to 96% (morphology and molecular) of the individuals assigned to a known tree taxon. CONCLUSION/SIGNIFICANCE: We conclude that while DNA barcoding is an invaluable tool for detecting errors in identifications and for identifying plants at juvenile stages, its limited ability to identify collections will constrain the practical implementation of DNA-based tropical plant biodiversity programs.
Pubmed
Web of science
Open Access
Oui
Création de la notice
14/12/2009 11:33
Dernière modification de la notice
20/08/2019 15:26
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