Sequential mechanism of solubilization and refolding of stable protein aggregates by a bichaperone network.

Détails

ID Serval
serval:BIB_6C7982C5386E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Sequential mechanism of solubilization and refolding of stable protein aggregates by a bichaperone network.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Goloubinoff P., Mogk A., Ben-Zvi A.P., Tomoyasu T., Bukau B.
ISSN
0027-8424 (Print)
ISSN-L
0027-8424
Statut éditorial
Publié
Date de publication
1999
Volume
96
Numéro
24
Pages
13732-13737
Langue
anglais
Résumé
A major activity of molecular chaperones is to prevent aggregation and refold misfolded proteins. However, when allowed to form, protein aggregates are refolded poorly by most chaperones. We show here that the sequential action of two Escherichia coli chaperone systems, ClpB and DnaK-DnaJ-GrpE, can efficiently solubilize excess amounts of protein aggregates and refold them into active proteins. Measurements of aggregate turbidity, Congo red, and 4,4'-dianilino-1, 1'-binaphthyl-5,5'-disulfonic acid binding, and of the disaggregation/refolding kinetics by using a specific ClpB inhibitor, suggest a mechanism where (i) ClpB directly binds protein aggregates, ATP induces structural changes in ClpB, which (ii) increase hydrophobic exposure of the aggregates and (iii) allow DnaK-DnaJ-GrpE to bind and mediate dissociation and refolding of solubilized polypeptides into native proteins. This efficient mechanism, whereby chaperones can catalytically solubilize and refold a wide variety of large and stable protein aggregates, is a major addition to the molecular arsenal of the cell to cope with protein damage induced by stress or pathological states.
Mots-clé
Bacterial Proteins/metabolism, Escherichia coli Proteins, HSP40 Heat-Shock Proteins, HSP70 Heat-Shock Proteins/metabolism, Heat-Shock Proteins/metabolism, Heating, Malate Dehydrogenase/metabolism, Molecular Chaperones/metabolism, Protein Denaturation, Protein Folding, Solubility, Substrate Specificity
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 21:02
Dernière modification de la notice
20/08/2019 15:26
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