Chronic in vivo hypoxia in various organs: hypoxia-inducible factor-1alpha and apoptosis

Details

Serval ID
serval:BIB_6C072555B0BD
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Chronic in vivo hypoxia in various organs: hypoxia-inducible factor-1alpha and apoptosis
Journal
Biochemical and Biophysical Research Communications
Author(s)
Bianciardi  P., Fantacci  M., Caretti  A., Ronchi  R., Milano  G., Morel  S., von Segesser  L., Corno  A., Samaja  M.
ISSN
0006-291X
Publication state
Published
Issued date
04/2006
Peer-reviewed
Oui
Volume
342
Number
3
Pages
875-80
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Apr 14
Abstract
We studied the in vivo persistence of hypoxia-inducible factor-1alpha (HIF-1alpha), main transducer of hypoxia, the differential response in organs exposed to the same degree of hypoxemia and the relationship with apoptosis. We measured HIF-1alpha (immunohistochemistry peroxidase and Western blot) and apoptosis (TUNEL) in heart, liver, kidney, gastrocnemius, and brain of rats exposed to chronic normobaric hypoxia (10% O2) or normoxia (21% O2) for 2 weeks. Despite same arterial O2 pressure and increased hemoglobin concentration (219 +/- 5 vs. 124 +/- 4 g/L), the organs responded differently. While marked in brain, muscle, and kidney cortex, HIF-1alpha was undetectable in heart and liver. In kidney medulla, HIF-1alpha was high in both normoxia and hypoxia. By contrast, apoptosis was marked in heart, slight in kidney medulla, and undetectable in other organs. We conclude that the HIF-1alpha response to chronic hypoxia can be a sustained phenomenon, but not in all organs, and that apoptosis responds differently from HIF-1alpha.
Keywords
Animals *Apoptosis Brain/*metabolism Cell Hypoxia/*physiology Hypoxia-Inducible Factor 1, alpha Subunit/*metabolism Kidney Cortex/*metabolism Muscles/*metabolism Organ Specificity Rats Rats, Sprague-Dawley
Pubmed
Web of science
Create date
18/01/2008 14:38
Last modification date
20/08/2019 14:26
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