Transient DNA Occupancy of the SMC Interarm Space in Prokaryotic Condensin.

Détails

ID Serval
serval:BIB_6B7EB2A633CA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Transient DNA Occupancy of the SMC Interarm Space in Prokaryotic Condensin.
Périodique
Molecular cell
Auteur(s)
Vazquez Nunez R., Ruiz Avila L.B., Gruber S.
ISSN
1097-4164 (Electronic)
ISSN-L
1097-2765
Statut éditorial
Publié
Date de publication
25/07/2019
Peer-reviewed
Oui
Volume
75
Numéro
2
Pages
209-223.e6
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Multi-subunit SMC ATPases control chromosome superstructure and DNA topology, presumably by DNA translocation and loop extrusion. Chromosomal DNA is entrapped within the tripartite SMCkleisin ring. Juxtaposed SMC heads ("J heads") or engaged SMC heads ("E heads") split the SMCkleisin ring into "S" and "K" sub-compartments. Here, we map a DNA-binding interface to the S compartment of E heads SmcScpAB and show that head-DNA association is essential for efficient DNA translocation and for traversing highly transcribed genes in Bacillus subtilis. We demonstrate that in J heads, SmcScpAB chromosomal DNA resides in the K compartment but is absent from the S compartment. Our results imply that the DNA occupancy of the S compartment changes during the ATP hydrolysis cycle. We propose that DNA translocation involves DNA entry into and exit out of the S compartment, possibly by DNA transfer between compartments and DNA segment capture.
Mots-clé
DNA loop extrusion, MukB, Rad50, SMC, Smc5, SmcScpAB, chromosome condensation, cohesin, condensin, kleisin
Pubmed
Web of science
Création de la notice
17/06/2019 21:44
Dernière modification de la notice
23/10/2019 5:13
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