Cytosolic N6AMT1-dependent translation supports mitochondrial RNA processing

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serval:BIB_6B56F28647D9
Type
Article: article from journal or magazin.
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Publications
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Title
Cytosolic N6AMT1-dependent translation supports mitochondrial RNA processing
Journal
bioRxiv
Author(s)
Foged Mads M., Recazens Emeline, Chollet Sylvain, Lisci Miriam, Allen George E., Zinshteyn Boris, Boutguetait Doha, Münch Christian, Mootha Vamsi K., Jourdain Alexis A.
Publication state
Published
Issued date
02/07/2024
Language
english
Abstract
Mitochondrial biogenesis relies on both the nuclear and mitochondrial genomes, and imbalance in their expression can lead to inborn error of metabolism, inflammation, and aging. Here, we investigate N6AMT1, a nucleo-cytosolic methyltransferase that exhibits genetic co-dependency with mitochondria. We determine transcriptional and translational profiles of N6AMT1 and report that it is required for the cytosolic translation of TRMT10C (MRPP1) and PRORP (MRPP3), two subunits of the mitochondrial RNAse P enzyme. In the absence of N6AMT1, or when its catalytic activity is abolished, RNA processing within mitochondria is impaired, leading to the accumulation of unprocessed and double-stranded RNA, thus preventing mitochondrial protein synthesis and oxidative phosphorylation. Our work sheds light on the function of N6AMT1 in protein synthesis and highlights a cytosolic program required for proper mitochondrial biogenesis.
Create date
16/07/2024 13:56
Last modification date
17/09/2024 6:20
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